Biotechnology and Its Applications - 2
Biotechnology and its Application - 2
Questions
Match the biologists given in Column I with their specific fields of achievements given in Column II.
| Column I | Column II |
| A. Alec Jefferey | i. Cytogenetics and radiation genetics in plants |
| B. M. S. Swaminathan | ii. Function of DNA ligase |
| C. Eli Lily | iii. DNA finger printing technology |
| D. Mertz and Devis | iv. Preparation of humulin |
- A – ii, B – i, C – iv, D – iii
- A – iii, B – i, C – iv, D – ii
- A – iv, B – iii, C – ii, D – i
- A – ii, B – iv, C – i, D – iii
Match the tumour suppressor genes given in Column I with their cancers in Column II.
| Column I | Column II |
| A. APC gene | i. Kidney carcinoma |
| B. RB gene | ii. Neurofibroma |
| C. VHL gene | iii. Colorectum carcinoma |
| D. NF-1 gene | iv. Retinoblastoma |
- A – iv, B – iii, C – i, D – ii
- A – iii, B – iv, C – ii, D – i
- A – iii, B – i, C – iv, D – ii
- A – iii, B – iv, C – i, D – ii
- A – ii, B – iii, C – i, D – iv
Karyotyping can be done from
- only the sex cells in our body
- any cells in our body
- only the embryonic cells in our body
- only the uterus cells in our body
By analysing a number of somatic cell hybrid lines for enzyme activities and their human chromosome constitution, the doctors can find out
- the number of chromosomes in the human genome
- the number of human chromosomes in each cell
- the location of gene on the chromosome that produces a specific enzyme
- the number of ribosomes in each cell
Directions: The following question has four choices, out of which ONLY ONE is correct.
Testing an individual for HLA haplotype to determine risk of an autoimmune disease would be an example of
- diagnostic tests
- screening tests
- predispositional test
- predictive testing
What is the first step in the southern blot technique?
- Denaturation of DNA on gel for hybridisation with specific probe
- Production of a group of genetically identical cells
- Digestion of DNA by restriction enzyme
- Isolation of DNA from a nucleated cell, such as the one from a scene of crime.
OCT, XAM and CAM are
- plasmids used in DNA probe
- plasmids helpful in bioremediation
- plasmids used to produce disease-resistant plants
- plasmids to promote conjugation
- none of the above
Which of the following is NOT a method applied in humans' genetic analysis?
- Karyotyping
- Pedigree analysis
- RFLP analysis
- Test cross
Kohler and Milstein developed biotechnology for
- monoclonal antibodies
- steroid synthesis
- immobilisation of enzymes
- myeloma
The mass of undifferentiated cells is called
- callus
- explant
- suspension culture
- leaven
A 10 nucleotide sequence, i.e. RNA primer is constructed by
- primase
- DNA ligase
- helicase
- topoisomerase
The enzyme used to cut the DNA molecule at specific site is
- restriction endonuclease
- DNA-ligase
- DNA-polymerase
- RNA-polymerases
Directions: Following question consists of two statements, one labelled as ‘Assertion A’ and the other labelled as ‘Reason R’. You are to examine these statements carefully and decide if the Assertion (A) and the Reason (R) are individually true and if so, whether the Reason is a correct explanation for Assertion. Select your answer from the codes given below.
(A) : DNA ligase is used as a fusogenic agent in protoplasmic fusion technology.
(R) : Fusogenic agent will promote the fusion of protoplasts.
- Both (A) and (R) are true and (R) is the correct explanation of (A).
- Both (A) and (R) are true and (R) is not the correct explanation of (A).
- (A) is true, but (R) is false.
- (A) is false, but (R) is true.
Which of the following statements is true about complementary DNA (cDNA)?
- It is the “other strand” of a DNA.
- It does not need any template for its synthesis.
- It is the base sequence of the vector DNA.
- It is produced by reverse transcription.