Mass Spectrometry

Mass Spectrometry involves ionizing molecules and measuring the mass-to-charge ratio of the resulting ions, providing information about their molecular weight and structure.

Electrospray Ionization (ESI) is a soft ionization technique that gently transfers ions from a liquid sample into the gas phase, preserving their molecular integrity.

The mass analyzer separates ions based on their mass-to-charge ratio, allowing for the identification and characterization of different compounds.

FT-ICR Mass Analyzers achieve exceptional mass-resolving power by measuring the frequency of ion cyclotron motion in a magnetic field.

The detector measures the abundance of ions, providing information about the relative concentrations of different compounds in a sample.

LC-MS is commonly employed for analyzing large biomolecules due to its ability to separate complex mixtures and provide detailed structural information.

Dissociation refers to the fragmentation of ions in Mass Spectrometry, which can occur through various mechanisms such as collision-induced dissociation (CID) or electron-capture dissociation (ECD).

ICP-MS is specifically designed for elemental analysis, allowing for the determination of the elemental composition of various materials.

Sample preparation techniques aim to convert the sample into a form that is compatible with the Mass Spectrometry analysis, ensuring efficient ionization and separation of the analytes of interest.

SIMS is a surface-sensitive Mass Spectrometry technique that allows for the analysis of the elemental and molecular composition of solid surfaces.

The mass-to-charge ratio (m/z) is a fundamental property of an ion, representing the ratio of its mass to its charge.

GC-MS is commonly used for analyzing volatile compounds due to its ability to separate and identify volatile analytes in complex mixtures.

Tandem Mass Spectrometry involves fragmenting selected ions and analyzing the resulting daughter ions, providing additional structural information about the parent molecule.

LC-MS is widely used for analyzing non-volatile compounds due to its ability to separate and identify non-volatile analytes in complex mixtures.

Ionization refers to the process of converting a neutral molecule into an ion by adding or removing electrons.