Questions
Which technique is used to introduce foreign genes into plant cells?
- Agrobacterium-mediated transformation
- Electroporation
- Microinjection
- All of the above
What is the role of the Ti plasmid in Agrobacterium-mediated transformation?
- It carries the genes necessary for DNA transfer
- It integrates into the plant genome
- It produces opines
- All of the above
Which enzyme is used to cut DNA at specific sequences?
- Restriction endonuclease
- DNA ligase
- RNA polymerase
- Reverse transcriptase
What is the purpose of a selectable marker gene in plant transformation?
- To confer resistance to antibiotics
- To allow for the identification of transformed cells
- To enhance the expression of the foreign gene
- All of the above
Which plant hormone is responsible for promoting cell division and shoot formation?
- Auxin
- Cytokinin
- Gibberellin
- Abscisic acid
What is the process of transferring genes from one organism to another called?
- Genetic engineering
- Recombinant DNA technology
- Gene therapy
- All of the above
Which technique is used to amplify a specific region of DNA?
- Polymerase chain reaction (PCR)
- Southern blotting
- Northern blotting
- Western blotting
What is the role of a promoter in gene expression?
- It binds RNA polymerase
- It enhances the expression of the gene
- It terminates transcription
- All of the above
Which technique is used to separate DNA fragments based on their size?
- Gel electrophoresis
- Chromatography
- Centrifugation
- Spectrophotometry
What is the role of a terminator in gene expression?
- It binds RNA polymerase
- It enhances the expression of the gene
- It terminates transcription
- All of the above
Which plant hormone is responsible for promoting root growth and development?
- Auxin
- Cytokinin
- Gibberellin
- Abscisic acid
What is the process of transferring a gene from one organism to another called?
- Gene transfer
- Genetic engineering
- Recombinant DNA technology
- All of the above
Which technique is used to detect the expression of a gene?
- Northern blotting
- Southern blotting
- Western blotting
- All of the above
What is the role of an enhancer in gene expression?
- It binds RNA polymerase
- It enhances the expression of the gene
- It terminates transcription
- All of the above
Which plant hormone is responsible for promoting seed germination and seedling growth?
- Auxin
- Cytokinin
- Gibberellin
- Abscisic acid