Light dependent or hill reaction - class-XII

In cyclic phosphorylation, oxygen is not evolved as the by-product where as, oxygen is evolved as a by-product during non-cyclic phosphorylation. Calvin cycle is the second stage of photosynthesis in which carbon atoms from carbon dioxide are combined, using the energy in ATP and NADPH, to make glucose.

The light reaction takes place in thylakoid discs. There, water is oxidized and oxygen is released. The hydrogen is accepted by $NADP$ and hence get reduced to $NADPH _2$. 

Photolysis is the process of breakdown of water molecule in the presence of sunlight. It results in the release of oxygen and hydrogen. It is also called as photodecomposition. It occurs during photosynthesis in a series of light-driven oxidation events. In this, water absorbs photons and the energy released during this process drives oxidation processes induced by light. 

The light reaction of the photosynthesis starts when the chlorophyll loses its electron after absorbing the light. In order to maintain the continuity of the reaction, there is a requirement of an oxidizable substance that can donate its electron to the chlorophyll. Water acts as the electron donor through a process called photolysis of water. In this process, the oxygen is also released as a by-product.

Phosphorylation refers to the process of formation of ATP. During respiration, the oxidation of NADH and FADH$ _{2}$ results in ATP formation, hence called oxidative phosphorylation. In photosynthesis, the photosystem II is involved in cyclic photophosphorylation. 

The acidic lumen of the thylakoids will have a higher concentration of H$^{+}$ ions. When these are transferred to basic pH of 8, the H$^{+}$ will tend to move outside due to their concentration gradient, which is higher in the lumen and lower in the surrounding. The thylakoids bear ATP synthases that will use the proton motive force H$^{+}$ ions and will make the ATP. So, an isolated chloroplast is capable of synthesizing the ATP when the lumen is made acidic and the outer medium is made alkaline.

Light reaction or photochemical phase is called as photophosphorylation or Hill's reaction. It includes non-cyclic phosphorylation and cyclic phosphorylation. During this reaction, the light is absorbed by the chlorophyll and water splits into H$ _2$ and 1/2O$ _2$. Oxygen is released as the byproduct of the reaction along with formation of carbohydrate. Along with oxygen, ATP and NADPH are also formed.

The events occurring in the light reaction of the photosynthesis are:

• The (light) compensation point is the light intensity on the light curve where the rate of photosynthesis exactly matches the rate of cellular respiration.
•  When the rate of photosynthesis equals the rate of respiration or photorespiration, the compensation point occurs.
  
• The compensation point is reached during early mornings and late evenings.
• Hence This condition occurs usually in evening and morning is not correct regarding light compensation point.
• So, the correct answer is 'Option A'.

The light-dependent reactions take place on the thylakoid membranes. The inside of the thylakoid membrane is called the lumen, and outside the thylakoid membrane is the stroma, where the light-independent reactions take place. Thylakoids are arranged in stacks called grana.
The chloroplast matrix is called the stroma and contains enzymes that catalyze the light-independent reactions of photosynthesis. But stroma is not the site for light reactions. 
Stromal lamellae connect two or more grana to each other. In this way, the lamellae act as a skeleton of the chloroplast, maintaining efficient distances between the grana, thereby maximizing the overall efficiency of the chloroplast. 
Each chloroplast is enclosed by (surrounded) a chloroplast envelope or unit membrane consisting of three layers. Overall the chloroplast envelope is semi-permeable. It is permeable to glucose molecules and certain ions including Fe$^{2+}$and Mg$^{2+}$, and oxygen and carbon dioxide.

In photosynthesis, energy is transferred in small energy steps. Photosynthesis is the primary energy source for most of the biosphere. Even though photosynthetic light harvesting complexes display great variety in their design and function, many of them are membrane proteins that comprise of pigment antenna arrays, which absorb light and transfer the resulting electronic excitation to a reaction center, which in turn converts this excitation energy to a charge gradient across the membrane and help to synthesise ATP. This energy is utilized in dark phase for synthesizing glucose.

Translocation is the process of movement of nutrients from leaves to other parts of plant. During the process of photosynthesis, carbohydrates (sugars) specifically sucrose is synthesized in the cytosol of cell and then translocated to other demanding parts of plant. Sucrose is complex, less reactive and energy efficient sugar so, angiosperms and other plants translocate sugar in the form of sucrose.

The light reaction takes place in the grana of the chloroplast. This is also known as the cyclic and non-cyclic form of photophosphorylation in which the ATP and NADPH + H$^+$ are produced. In the non-cyclic photophosphorylation, there is splitting of the water molecules which results in the production of oxygen molecule. 

Photophosphorylation refers to the use of light energy from photosynthesis to ultimately provide the energy to convert ADP to ATP, thus replenishing the universal energy currency in living things. Light generally provides energy to form the bonds. It is an endothermic reaction.

In photosynthesis, only a small fraction of the total incident solar energy is converted into chemical energy. Scientific literature typically cites values in the range of 1% to 5% for overall efficiency, but specific textbook contexts sometimes use 35% as a theoretical maximum for specific wavelengths or specific reaction steps.

In light-dependent reactions, the energy from sunlight is absorbed by chlorophyll and converted into chemical energy in the form of electron carrier molecules like ATP and NADPH.

Light phase is also known as a photochemical phase because it is a light dependent phase. During this phase, the formation of high-energy chemical intermediates ATP and NADPH$ _{2}$ takes place. ATP and NADPH$ _{2}$ are the assimilatory power of photosynthesis. Light phase also includes light absorption, water splitting and release of oxygen. Two photosynthetic units called Photosystem I and Photosystem II are also involved in light phase. Light phase consists of two photochemical reactions, i.e. non-cyclic photophosphorylation and cyclic photophosphorylation.

In photosynthesis, the light-dependent reactions take place on the thylakoid membranes. The light-independent reactions take place in the stroma. The thylakoid membrane contains some integral membrane protein complexes that catalyze the light reactions. There are four major protein complexes in the thylakoid membrane Photosystem II (PSII), Cytochrome b6f complex, Photosystem I (PSI), and ATP synthase. These four complexes work together to ultimately create the products ATP and NADPH.

Light reactions of photosynthesis which takes place in thylakoids membranes of chloroplast utilize sunlight and water to produce oxygen, ATP and NADPH.

Photosynthesis is a process in which phosphorylation occurs. Phosphorylation is a process in which phosphate group is added, and in the case of photosynthesis it happens in the presence of light, thus called as 'photophosphorylation'. ADP is converted to ATP, the energy currency as the main product of this process. In photosynthesis, cyclic and non-cyclic photophosphorylation takes place in the light phase and generate ATP and NADPH.

Photoluminescence is the phenomenon of light emission after absorption of photons in the form of electromagnetic radiation. Chemiluminescence is the phenomenon of emission of light from a chemical reaction. Phosphorescence is a type of photoluminescence which is related to fluorescence. It does not re-irradiate the absorbed light immediately like fluorescence. Fluorescence is the phenomenon of re-irradiation of light absorbed light or electromagnetic radiation.

A) ADP and ${ H } _{ 2 }O$ are required in addition to NADP.

The process that converts light energy into chemical energy takes place in a multi-protein complex called a photosystem. Two types of photosystems are embedded in the thylakoid membrane:- photosystem II ( PSII) and photosystem I (PSI). Each photosystem plays a key role in capturing the energy from sunlight by exciting electrons. These energized electrons are transported by “energy carrier” molecules, which power the light-independent reactions.

The first and foremost step of photosynthesis is the excitation of chlorophyll molecule which occurs with the absorption of light. This reaction is photochemical reaction since involves light and results in chemical change by transfer of electrons. The pigments present in the photosystems absorb photon of light. The photons excite electrons in the chlorophyll which then move through the electron transport chain and results in the production of carbohydrates and energy packets NADPH, ATP with the release of oxygen.

The Hill reaction implies that the light-dependent reaction of photosynthesis is a result of a series of redox reactions and a suitable terminal electron acceptor is required for that reaction to occur. Hence, solar energy is converted to chemical energy by the reduction of NADP to NADPH.

During the first stage of photosynthesis, called the light-dependent reaction, sunlight excites the electrons in the chlorophyll pigment. The organism uses this energy to create the energy carrier molecules ATP and NADPH, which are crucial for carbon fixing during the second stage.

• Moles = Weight in gms/molecular weight

• In same way moles of H2O = 216/18 = 12 moles.

Photosynthesis is the process by which plant utilizes the energy obtained from sunlight to produce glucose from carbon dioxide and water. During this process, oxygen is released as the by product. It takes place in leaf cells. Leaf cells contain chloroplasts. The chloroplasts contain green pigment chlorophyll which prepares the food during photosynthesis.

When the light hits the chloroplast, the chemiosmotic potential is established by thylakoid membrane. The carriers in the electron transport chain use some of the electron’s energy to actively transport protons from the stroma to the lumen. During photosynthesis, the lumen becomes acidic, as low as pH 4, compared to pH 8 in the stroma. Therefore answer B is correct.

In plants, the light reactions take place in the thylakoid membranes of organelles called chloroplasts. The light-dependent reactions use light energy to make two molecules needed for the next stage of photosynthesis: the energy storage molecule ATP and the reduced electron carrier NADPH. 

During the first stage of photosynthesis, called the light-dependent reaction, sunlight excites the electrons in the chlorophyll pigment. The organism uses this energy to create the energy carrier molecules ATP and NADPH, which are crucial for carbon fixing during the second stage. 

Phosphorylation in the electron-transport chain in the mitochondrion.

Green pigments are located within the chloroplasts of plant. Chlorophyll a can participate directly in light reaction which convert solar energy to chemical energy.

The ATP and NADPH from the light-dependent reactions are used to make sugars in the next stage of photosynthesis. The net result of this reaction is the production of 2 ATP and 9 NADPH and the photolysis of water. Light is absorbed and the energy is used to drive electrons from water to generate NADPH and to drive protons across a membrane. These protons return through ATP synthase to make ATP.

When photosystem II absorbs light, electrons in the reaction-centre chlorophyll are excited to a higher energy level and are trapped by the primary electron acceptors. Photoexcited electrons travel through the cytochrome b6f complex to photosystem I via an electron transport chain set in the thylakoid membrane. The light-dependent reactions use light energy to make two molecules needed for the next stage of photosynthesis: the energy storage molecule ATP and the reduced electron carrier NADPH. In plants, the light reactions take place in the thylakoid membranes of organelles called chloroplasts.

The thylakoid membranes have pigment system, electron transport proteins as well as the ATP synthases. When the transfer of electron down the transport proteins occurs, many H$^{+}$ are transported into the thylakoid lumen from the stroma. This high concentration of H$^{+}$ is then used by the ATP synthases to make ATP by transferring these H$^{+}$ out of the thylakoid lumen in the stroma. The punctured thylakoid will not have an intact thylakoid lumen, so the concentration of the H$^{+}$ ins will not be developed. As a result, ATP synthase will not function and so ATP will not be synthesized. 

The overall function of light-dependent reactions is to capture the energy from the light for the generation of ATP and NADPH molecules. The dark reactions of the C$ _{3}$ cycle utilize the energy from short-lived electronically excited carriers to convert CO$ _{2}$ & H$ _{2}$O into glucose catalyzed by the enzyme RuBisCo, and this constitutes carbon fixation and makes it the enzymatic phase.

Chlorophyll present in the plants can absorb the the wavelength of both blue and red regions of VIBGYOR which ranges from 400-500 nm and 600-700 nm respectively. It reflects the green light range of 500-600 nm hence the leaves appear green. 

The light-dependent reactions start in photosystem II (PSII). When the pigment in the reaction centre of PS II i.e, P$ _{680}$ absorbs a photon, an electron in this molecule gets excited and transferred to a primary electron acceptor, Pheophytin and then go through molecules in a series of redox. The electron flow goes from PSII to cytochrome b6f complex than to PSI. In PSI, the electron is finally accepted by NADP. Thus photosystem I in light-dependent reaction directly transfers electrons to NADP.

PS I: This system produces a strong reductant which reduces ${NADP}^+$ to $NADPH$.

A) Carbon dioxide is reduced, not oxidised. 
B) Water is oxidised to form protons and release oxygen. ${ H } _{ 2 }O\longrightarrow { O } _{ 2 }+{ 2H }^{ + }$

Green plants are green because they contain a pigment called chlorophyll as in the absorption spectra, chlorophyll absorbs light in the red (long wavelength) and the blue (short wavelength) regions of the visible light spectrum. The green light is not absorbed but reflected, making the plant appear green. An absorption spectrum shows all the light typically absorbed by a leaf. An action spectrum, meanwhile, shows all the light that is actually used for photosynthesis. The similarity of the action spectrum of photosynthesis and the absorption spectrum of chlorophyll tells us that chlorophyll is the most important pigments in the process. The spectra are not identical, though, because carotenoid, which absorbs strongly in the blue, play a role as well.

In the year 1937 and 1939, Robert hill studied the light dependent reaction and revealed that oxygen evolved during photosynthesis came from water. In his experiment, he used Dichlorophenolindophenol (DCPIP) as a redox dye to measure the rate of photosynthesis. The dye is blue in oxidized state with a maximal absorption at 600 nm whereas in reduced, DCPIP is colourless.

Photosynthesis
takes place in the green leaves of plants and other green part of plants like
stem etc. The most active photosynthetic tissue in higher plants is the
mesophyll of leaves. Mesophyll cells have many chloroplasts, which contain the
specialised light absorbing green pigments, the chlorophylls. When chlorophyll
absorbs light, it gets excited and emits electrons. Chlorophyll a is the
primary photosynthetic pigment. It shows bright or blue green colour in
chromatogram. Chlorophyll a is directly involved in light reaction of
photosynthesis. It is present in the reaction centres of Photosystem I and
Photosystem II which absorbs light energy of longer wavelength. These centres
can release electron upon absorption of energy.

Photosynthesis takes place in the green leaves of plants and other green parts of plants like stem etc. When chlorophyll absorbs light, it gets excited and emits electrons. These chlorophylls are found in photosynthetic units called Photosystem I and Photosystem II. Each unit has a specific reaction center which contains pigment molecules. These molecules absorb light of different wavelengths and emit electrons. These electrons are picked up by an electron acceptor which passes them to an electron transport system of cytochromes.