Structure of dna - class-XI

Two polynucleotide chains of DNA are wound around the same axis and are held together by complementary base pairing between nitrogenous bases of two strands in the same plane. Adenine of one strand forms two hydrogen bonds with thymine of other strand and guanine forms three hydrogen bonds with cytosine.  The two bases forming hydrogen bonds to joins the polynucleotide strands together are present in the same plane. Thus, the correct answer is A.

As per Watson and Crick standard base pairing rules, adenine forms double hydrogen bond with thymine and guanine forms triple hydrogen bond with cytosine. Hence, content of adenine is equal to content of thymine and content of guanine is equal to content of cytosine.

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids like DNA and RNA. The building blocks of nucleic acids, nucleotides are composed of a nitrogenous base, a five-carbon sugar-ribose or deoxyribose, and at least one phosphate group. Thus, a nucleoside plus a phosphate group yields a nucleotide. The distance between two nucleotide pairs in DNA is 0.34nm.

Nucleosomes are fundamental organizational units of chromatin which appear as “beads-on-a-string” arrangement. Here beads represent complexes of histones and DNA. Each bead contains a core of histone octamer: two copies each of H2A, H2B, H3, and H4; out of 200 bp DNA, 146 bp DNA is bound tightly around the histone core and the remainder serve as linker DNA between nucleosomal beads. The H1 histone associates with the linker DNA to pack adjacent nucleosomes together and forms a compact 30-nm chromatin fiber. Thus, the correct answer is A.

RNA and DNA are genetic materials. Cytosine, Thymine, Guanine, and Uracil are all nitrogenous bases. They are present in RNA. Uracil is replaced by adenine in DNA. Arginine is an amino acid which is basic in nature. It is the building block of protein. 

Purines and pyrimidines, make up the two groups of nitrogenous base which plays on important role in structural backbone of DNA. Adenine and guanine are purines, while cytosine and thymine are pyrimidines. Purines pairs with pyrimidines, by formation of H-bonds. Adenine attaches to thymine, by two hydrogen bonds, while cytosine and guanine forms three hydrogen between them.

Genetic information is carried in the linear sequence of nucleotides in DNA. Each molecule of DNA is a double helix formed from two complementary strands of nucleotides held together by hydrogen bonds between G-C and A-T base pairs.

According to the Watson Crick double helical model of DNA, the diameter of DNA molecule is 20 angstrom. Hence, the distance between two DNA strand is 20 angstroms.
So, the correct answer is option A.

DNA is acidic. For its compaction, it requires basic (histone) proteins. The histone proteins, are positively charged and occupy the major grooves of DNA at an angle of 30° to helix axis. 

The distance between two complementary strands always remains the same because there are hydrogen bonds between the nitrogen bases of two strands restricting movement. The bonds are formed correctly on the opposite ie the bonds are between purines and pyrimidines.

DNA has a double stranded structure where, the purines are linked to pyrimidines with the help of hydrogen bonds. Adenine is linked to thymine by two hydrogen bonds while, cytosine is linked to guanine by three hydrogen bonds.

According to the base pair rule, purine pairs with pyrimidine. Hence, guanine pairs with cytosine by formation of three hydrogen bonds.

Answer is option B i.e. "Weak hydrogen"
DNA is made up of nucleotide. Nucleotides join up with one another based on chemical compatibility of their nitrogen bases that are held together by weak hydrogen bond. In DNA, this hydrogen bond helps in stabilizing the nucleic acid structure.

Species that are very closely related have very similar DNA with only minor differences. They are related through a common ancestor. 

In DNA, purines pairs with pyrimidines. Adenine pairs with Thymine with double hydrogen bond and Guanine pairs with Cytosine with the help of triple hydrogen bond.

Both sense and antisense strands are present in DNA as these act as antisense strand acts as a template during transcription and sense strand has the same sequence as mRNA in the nucleus.

The structure of DNA was deciphered by Watson and Crick in 1953 on the basis of X-ray diffraction data generated by Wilkins, Astbury and Franklin. Wilkins, Watson and Crick were awarded Nobel prize for their model in 1962.
So, the correct answer is option D.

DNA duplex of Watson and Crick is right handed and is called as B-DNA. Z-DNA is left handed double helix, which contains zigzag backbone of phosphodiester bonds and 12 base pairs per turn. The diameter of Z-DNA is 18 $\mathring A$ as against B-DNA diameter of 20 $\mathring A$.
So, the correct answer is option D.

Surface area to volume ratio increases as the size of an object decreases. DNA, being a molecular structure, has a significantly higher surface area to volume ratio compared to cellular structures like red blood cells or macroscopic objects like hair and nails.

Glucose (C$ _6$H$ _1$$ _2$O$ _6$), Deoxyribose (C$ _5$H$ _1$$ _0$O$ _4$), Lactose (C$ _1$$ _2$H$ _2$$ _2$O$ _1$$ _1$), Ribose (C$ _5$H$ _1$$ _0$O$ _5$)

B-DNA is physiologically the most important form of DNA. The double helical secondary structure model of DNA described by Watson and Crick was the B-DNA. As per the original model, it contains 10 bp per turn but later measurements showed that it contains 10.5 base pairs per turn. Z DNA is the zigzag DNA. It is left handed helix. There are 12 base pair per turn, with a rise of 0.38 nm per base pair.
So, the correct answer is option B.

• DNA replication goes in the 5' to 3' direction because DNA polymerase acts on the 3'-oh of the existing strand for adding free nucleotides.
• Histones are rich in the basic amino acid residues lysines and arginines and forms histone octamer.
• Catalytic converters, having expensive metals namely platinum-palladium and rhodium as the catalysts, are fitted into automobiles for reducing the emission of poisonous gases.
• As the exhaust passes through the catalytic converter, unburnt hydrocarbons are converted into carbon dioxide and water, and carbon monoxide and nitric oxide are changed to carbon dioxide and nitrogen gas, respectively.
• Fermentation of idli or dosa batter is carried out largely by lactic acid bacteria, the same class of microorganisms that are found in ‘curd.’
  
• So the correct answer is " Template with 5′⟶3′ polarity shows continuous DNA replication ".

DNA is a macromolecule of high molecular weight made up of repeating units called nucleotides. Each nucleotide is made up of

DNA replication, is the process of producing two identical replicas from one original DNA molecule. This biological process, occurs in all living organisms and forms the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication.

The purine & pyrimidine pairs of complementry strands of DNA are held together by hydrogen bonds. A set of five nitrogenous bases is used in the construction of nucleotides, which in turn build up the nucleic acids like DNA and RNA.

Purines and pyrimidines, make up the two groups of nitrogenous base
which plays on important role in structural backbone of DNA. Adenine and
guanine are purines, while cytosine and thymine are pyrimidines.
Purines pairs with pyrimidines, by formation of H-bonds. Adenine
attaches to thymine, by two hydrogen bonds, while cytosine and guanine
forms three hydrogen between them.

If DNA is subjected to high temperature, the hydrogen bonds between the nitrogenous bases break down and the two strands separate. This process is called as melting of DNA. More the content of guanine (G) and cytosine (C) in the DNA, higher will be the melting temperature because G forms three hydrogen bonds with C as against adenine (A) and thymine (T) which form two hydrogen bonds.
So, the correct answer is option A.

The C$ _1$ of pentose sugar in nucleotides is linked to the N1 or N9 position of pyrimidine or purine bases through a glycosidic bond. The syn and anti conformations, are the two conformational forms of nucleotides around this bond. The two forms are interconvertible by rotating the glycosidic bond without a need to break the bond.
So, the correct answer is option B.

The DNA duplex of Watson and Crick is right handed helix and is called the B-DNA. There are five more models (A-DNA, C-DNA, D-DNA, E-DNA and Z-DNA) have been proposed. The Z-DNA is left handed double helix which contains zigzag back and 12 base pairs per turn.
So, the correct answer is option A.

The base pairing confers a unique property to the polynucleotide chains. The chains are complimentary to each other, such that if the sequence of one strand is known then the sequence of other strand can be predicted. Also if each strand of DNA acts as a template, for the synthesis of a new strand, the two double stranded DNA molecule thus produced would be identical to the parent molecule. The bases in the two strands of a double stranded DNA molecule are paired through hydrogen bonds forming base pairs (bp). Adenine forms two hydrogen bonds with thymine from opposite strand and vice versa. Similarly cytosine is bonded with guanine from three H bonds. As a result, always a purine comes opposite to a pyrimidine. This generated approximately, equal distance between the two strands. If base order in one chain of DNA is "ATCGA" then the number of H bonds will be 2, 2, 3, 3 and 2 respectively for the given nucleotides to base pair. Thus, the total number of hydrogen bonds will be 12.

Wilkins X -ray diffraction showed the diameter of the DNA helix is around 20 $\mathring A$.  According to Maurice Wilkins, the structure of DNA of all species comprises two helical chains each coiled round the same axis, and each with a pitch of 34 angstroms and a radius of 10 angstroms. According to another study, when measured in a particular solution, the DNA chain measured 20 to 26 angstroms wide, and one nucleotide unit measured 3.3 long. Although each individual repeating unit is very small, DNA polymers can be very large molecules containing millions of nucleotides.
So, the correct answer is option B.

DNA is a polymer of deoxyribonucleotides. The deoxyribonucleotides are made up of phosphoric acid and deoxyribonucleosides. The deoxyribonucleosides are made up of deoxyribose sugars and nitrogenous bases. The deoxyribonucleotides are joined to each other with the help of 3', 5' phosphodiester bonds forming the backbone of single DNA chain. In a molecule of DNA, two such chains are present attached with each other with the help of hydrogen bonds between the nitrogenous bases of deoxyribonucleotides.

DNA molecule has uniform diameter due to specific base pairing between purine and pyrimidine. In DNA, the bases adenine and guanine form hydrogen bonds with their complementary pyrimidines; thymine and cytosine, respectively, this is called as complementary base pairing.

DNA is a double helical molecule made of two antiparallel strands of nucleotides which is linked by hydrogen bonds between complementary base pairs. DNA carries the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms. Adenine (A) pairs with Thymine (T) via two hydrogen bonds. Guanine (G) pairs with Cytosine (C) via three hydrogen bonds. Hence option A is correct.

The DNA strands runs anti-parallel, due to phosphodiester bonds. A phosphodiester bond occurs when exactly two of the hydroxyl groups in phosphoric acid react with hydroxyl groups on other molecules to form two ester bonds. Phosphodiester bonds are central to most life on Earth, as they make up the backbone of the strands of DNA. In DNA and RNA, the phosphodiester bond is the linkage between the 3' carbon atom of one sugar molecule and the 5' carbon atom of another, deoxyribose in DNA and ribose in RNA. Strong covalent bonds form between the phosphate group and two 5-carbon ring  two ester bonds. Due to this bond the DNA strands run anti-parallel.

DNA (Deoxyribonucleic Acid) is composed of two polynucleotide strands (the polymers of nucleotides), which form what looks like a ladder. The nitrogenous bases in DNA store the instructions for making polypeptide chains, essentially coding for every feature of the entire organism. The two polynucleotide strands run 'antiparallel' to each other, with nitrogenous bases projecting inwards. The term 'antiparallel' means that the strands run in opposite directions, parallel to one another. The antiparallel strands twist in a complete DNA structure, forming a double helix.

Nucleotides are formed by the combination of purines or pyrimidines (nitrogenous bases), sugars (pentose), and phosphates. Nucleosides lack the phosphate group, making option A the correct answer.

A DNA strand is complementary. This means adenine pairs with thymine and cytosine pairs with guanine in the opposite strand. The given strand has a sequence 5'-GAATTG-3'. Hence the complementary strand will have a sequence 5'-CAATTC-3'. Thus the correct answer is option A.

When DNA strands run in opposite direction one strand from 5 prime to 3 prime and another strand from 3 prime to 5 prime is called as antiparallel. The 5 prime end have phosphate in 5$^{th}$position of sugar and 3 prime end have a hydroxyl group on 3$^{rd}$ position of sugar. DNA strands are antiparallel because of the presence of hydrogen bond. Hydrogen bond binds the complementary bases (purine and pyrimidine)

The nitrogenous bases present in the DNA are purines and pyrimidines. The purines are adenine and guanine. The pyrimidines are thymine and cytosine. The purines are double ring structure while the pyrimidines are the single ring made up of carbon and nitrogen. Purines pair with the pyrimidines by hydrogen bonds. Adenine pairs with thymine by two hydrogen bond while the cytosine pairs with the guanosine by three hydrogen bonds.

DNA is very long. It is accommodated in the small-sized nucleus by super coiling. The enzyme topoisomerase helps in the supercoiling of the DNA which helps in packaging and some biological process. The super coiled DNA is uncoiled during the process of transcription and translation. During transcription the enzyme helicase cuts the phosphate backbone and separates the two super coiled DNA strands while the topoisomerase balances the strain and prevents the recoiling of the DNA.

Length of $3.4 nm $ includes $10$ base pairs.So, $68 nm $ will include $200$ base pairs that is $400$ bases.

Deoxyribose Nucleic Acid or DNA carries the genetic information in the form of small segments known as genes and is also the genetic material in various organisms including humans.

Although the discovery of the structure of DNA was attributed to Watson and Crick, a keynote player in helping them discover this structure was a scientist by the name of Rosalind Franklin. Rosalind Franklin, along with Francis Wilkins, worked on DNA applying X-ray crystallography to find out its structural properties. X-ray crystallography required the process of exposing a crystal specimen (DNA) to X-rays to determine the locations of the atoms in the “molecules that comprises basic unit of crystal called unit cell”. 

Adenine and Guanine are the two purines found in DNA and RNA. The pyrimidines of DNA are represented by thymine and cytosine. 

The DNA double helical structure has two different environments. The nitrogenous bases have an aromatic ring structure and are hydrophobic and interact with each other through base pair stacking interactions. At the same time, the back bone of DNA formed by phosphodiester bonds is hydrophilic and interacts with water. The nitrogenous bases of one nucleotide chain form hydrogen bonds with the nitrogenous bases present directly opposite them on the other chain. The hydrogen bonds are formed in polar aqueous environment. The adenine base pairs with thymine with two hydrogen bonds and guanine base pairs with cytosine with three hydrogen bonds.
So, the correct answer is option A.

According to the most widely accepted model of DNA structure, the distance between any two base pairs in DNA is approximately 3.4 angstroms. Thus for a DNA molecule having 10,000 base pairs; the length will be 10,000 x 3.4 or 34000 angstroms or 3400 nanometers.
So, the correct answer is option B.

In any double stranded DNA, adenine (A) pairs with a thymine (T) and Guanine (G) pairs with a cytosine (C).
Now, since each A always pairs with a T, the percentage of A and T will always be the same. Therefore, percentage of T will also be 17%.
So we can conclude that A+T=17+17=34%
The rest would account for G+C which would be 100-34=66%, thus G+C=66%.

Amino acid synthesis stops when any STOP codon is encountered by ribosome. There are total 3 stop codons, UAG, UGA, UAA. Codons are always read in triplets. So the given ribonucleotide sequence will be read as: AUG,GUG, UAG, and so on. Notice that the third codon (UAG) is a stop codon, so the protein synthesis will stop at the third codon itself. It doesn't matter if the 15th base is substituted by G or not, the given sequence will code for only two amino acids, AUG (methionine) and GUG (Methionine)

DNA cannot pass through a cell membrane as it is hydrophilic in nature whereas cell membrane is made up of lipid bilayer. 

• The feulgen test is a staining technique discovered by Robert Feulgen and used in histology to identify chromosomal material or DNA in cell specimens.
• The Feulgen test, is unique for DNA, is a Basic Fuchsin.
• It is used for demonstration of DNA (deoxyribonucleic acid) in tissue sections. 
• So, the correct answer is 'Basic fuchsin',   

Bonding between deoxyribose and base in pyrimidine nucleoside molecule is $1'-1'$ glycosidic linkage because 

Meselson-Stahl experiment -  DNA Replication is Semiconservative.