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DNA Fingerprinting - Class XII
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Fill up the blanks and select the correct option.
(i) EcoRI cuts the DNA between bases ___ only ____ when the sequence _____ is present in the DNA.
(ii) Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes respectively ____, ____ and ___.
(iii) Since DNA has a ____ charge, it moves towards the ____ of the electrophoretic chamber.
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A
(i) G A, GAATTC (ii) lysozyme, cellulase, chitinase negative/anode
💡 Explanation:
- EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA.
- Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes lysozyme, cellulase and chitinases respectively.
- Since DNA has a negative charge , it moves towards the anode of the electrophoretic chamber.