Biodiversity Conservation and Environmental Monitoring

A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA

Gene banks are a type of biorepository, which preserve genetic material. For plants, this could be by freezing cuttings from the plant, or stocking the seeds (e.g., in a seed bank). For animals, this is the freezing of sperm and eggs in zoological freezers until further need. With corals, fragments are taken which are stored in water tanks under controlled conditions.
Plant genetic material in a 'gene bank' is preserved at $-196^0$C in liquid nitrogen as mature seed (dry).

A biosphere reserve is an ecosystem with plants and animals of unusual scientific and natural interest. It is a title given by UNESCO to help protect the sites. The plan is to promote management, research and education in ecosystem conservation. This includes the 'sustainable use of natural resources'. 
In situ conservation is on-site conservation or the conservation of genetic resources in natural populations of plant or animal species, such as forest genetic resources in natural populations of tree species.

Genetic erosion is a process whereby an already limited gene pool of an endangered species of plant or animal diminishes even more, when individuals from the surviving population die off without getting a chance to meet and breed with others in their endangered low population. 

Germplasm is a term used to describe living genetic resources, such as seeds or tissue, maintained for the purpose of breeding, preservation, and other research uses. These resources may take the form of seed collections stored in seed banks, trees growing in nurseries, animal breeding lines maintained in animal breeding programs or gene banks, etc. Germplasm collections can range from collections of wild species to elite, domesticated breeding lines that have undergone extensive human selection.

Genetic diversity refers to the variety of genes within a species. Each species is made up of individuals that have their own particular genetic composition. Within a species there may also be discrete populations with distinctive genes. Speciation is the evolutionary process by which new biological species arise. The biologist Orator F. Cook was the first to coin the term 'speciation' for the splitting of lineages or "cladogenesis," as opposed to "anagenesis" or "phyletic evolution" occurring within lineages.
More diversity will be introduced to gene pool when speciation occurs; as a result of which more and more species will be produced.

Agrobacterium tumefaciens will transfer genes (T-DNA region of Ti plasmid) into the plant that produces its food or nutrient called opines (opines are not needed for plants). Apart from that, it also integrates genes for cell division and proliferation leading to crown gall disease, so that more number of cells result in more production of opines. Because of this reason it is called as a 'natural genetic engineer'.

Genetic diversity refers to the total number of genetic characteristics in the genetic makeup of the species. Genetic diversity serves as a way for populations to adapt to changing environments.
In order to capture the maximum genetic diversity of a species, recent, old, wild - all kind of varieties will be considered and taken into account and searched.
Thus, the correct answer is option (D),  'All of the above'.

Extinction of a particular animal or plant species occurs when there are no more individuals of that species alive anywhere in the world - the species has died out. When it does not exist at all , preserving it also not possible.

Genetic erosion is a process whereby an already limited gene pool of an endangered species of plant or animal diminishes even more when individuals from the surviving population die off without getting a chance to meet and breed with others in their endangered low population. Low genetic diversity in a population of wild animals and plants leads to a further diminishing gene pool, inbreeding and a weakening immune system and fast tracks that species towards eventual extinction. 

Wild varieties of plants must be conserved because they are of utmost value to plant breeders for producing improved and favourable varieties of plants by selecting useful genes from a wide range of their wild relatives. In other words, new and improved varieties of crops are derived from their wild relatives by genetic modification. Useful traits are like high yielding, disease resistant, stress resistant etc.

Germplasm is a living tissue from which new plants can be grown. It can be a seed or another plant part like a leaf, a piece of stem, pollen or even just a few cells that can be turned into a whole plant. Germplasm contains the information for a species genetic makeup, a valuable natural resource of plant diversity. 

Shoot tip culture is the culture of terminal part of shoot to a plant in in-vitro condition or in lab called as shoot tip culture. Mostly the shoot tip culture used for obtain disease free plant without genetically changes. The plant which affected by any contaminant mostly by viruses have saved by shoot tip culture, because shoot tip of any affected plant is less or free from contamination (viruses, bacteria, fungus). 

Vavilov worked on global genetic diversity of cultivated plants throughout his life, from which he developed several major theories that have played an important role in the development of botany, genetics and plant breeding. One such theory among several is "Theory of centres of origin of cultivated plants" (1920).

A genomic library is a collection of total genomic DNA from a single organism. This DNA is packed in a population of identical vectors for storage. Each vector contains a different insert of DNA. 

A gene library can be defined as a collection of living bacteria colonies that have been transformed with different pieces of DNA from the organism. These DNA fragments are the sources of the genes of interest. 

Genetic erosion is a process, whereby an already limited gene pool of an endangered species of plant or animal diminishes even more when individuals from the surviving population die off without getting a chance to meet and breed with others in their endangered low population. The term is sometimes used in a narrow sense, such as when describing the loss of particular alleles or genes, as well as being used more broadly, as when referring to the loss of varieties or even whole species.
Genetic erosion occurs because each individual organism has many unique genes which get lost when it dies without getting a chance to breed. Low genetic diversity in a population of wild animals and plants leads to a further diminishing gene pool inbreeding and a weakening immune system can then "fast track" that species towards eventual extinction.

DNA probes are stretches of single-stranded DNA used to detect the presence of complementary nucleotide sequences (target sequences) by hybridization. Genomic library comprises large number of genes in the form of different nucleotide sequences of DNA fragments and they can be selected with the help of DNA probes.

Bioindicators are living organisms such as plants, animals, and microorganisms that are used as indicators. They are used for knowing the different content of an ecosystem such as minerals, oxygen demand, air pollution. Lichens are used for knowing air pollution. Algae bloom indicates large amount of nitrates and phosphates in lakes and rivers.

Lichen is a symbiotic association between algae and fungi known as phycobiont and mycobiont component respectively. Lichens are very good pollution indicators as they do not grow in SO$ _2$ polluted environments.

A lot of pathogens are present in water bodies that are contaminated. One of these pathogens is coliform bacteria. This bacteria, like other pathogens, is an indicator of contamination in water bodies. This is because it has a similar reaction to purification and water treatment like other pathogens.

SO$ _2$ is produced during combustion of fossil fuels, refining of petroleum, smelting of sulphur containing ores, smog and acid rain. SO$ _2$ causes membrane damage, destruction of chlorophyll, necrosis and water soaked appearance. Lichens are the most sensitive organisms to SO$ _2$ pollution and do not grow in areas with high levels of sulphur dioxides. Hence, the correct answer is option C.