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History of photosynthesis - class-XII
Which scientists are related with light reaction and what is its site?
Chloroplasts contain an internal system of membranes called thylakoids. The thylakoids may be arranged in stacks called as grana. The thylakoid membranes not arranged in stacks and are in direct contact with chloroplast stroma are called as stroma lamellae. During photosynthesis, light reaction occurs in grana.
Hill conducted experiments on isolated chloroplasts, having some oxidants like ferricyanide and benzoquinone (Hill reagents). By placing them in sunlight, he noticed evolution of oxygen from water. Hill concluded that if there was hydrogen acceptor in the medium, it would have been reduced. Arnon in 1951, 1954, repeated the experiment with isolated chloroplast and concluded that -
(i) The hydrogen acceptor present in plants is NADP. It is reduced to $NADPH _2$.
(II) An ADP molecule is converted to ATP in light by utilizing inorganic phosphorous. This phenomenon is called as photophosphorylation.