Biochemistry, Metabolism and Bioenergetics
GATE LS Biochemistry: Metabolic pathways, enzyme kinetics, bioenergetics, and metabolic disorders
Questions
Which of the following statements is false about lactate synthesis?
- Lactose synthase has catalytic subunits.
- It occurs in mammalian tissue.
- α lactoalbumin allosterically inhibit lactose synthase.
- None of these
How many ATPs are generated in aerobic condition through glycolysis?
- 4
- 2
- 8
- 5
Glycolysis is the process that takes place in cytosol. In this reaction, glucose is broken down into two molecules of pyruvic acid. This reaction involves many enzyme-dependent steps. Name the enzyme responsible for the cleavage of Fructose 1,6-diphosphate (Glycolysis).
- Kinase
- Mutase
- Dehydrogenase
- Aldolase
Which of the following processes is the correct for the conversion of FAD?
- Malate formation
- Succinate formation
- Fumarate formation
- $\alpha$- Keto glutarate formation
Which of the following occurrs partially in mitochondria and partially in cytosol?
- Gluconeogenesis
- Calvin cycle
- Glycolysis
- TCA Cycle
Which of the following is a competitive inhibitor?
- Malonate
- Fluoroacetate
- Fumarate
- All of the above
What happens if insulin decreases?
A. Gluconeogenesis is inhibited.
B. Glycolysis is stimulated.
C. Gluconeogenesis is stimulated.
D. Glycogenesis is stimulated.
- A, B, D
- B, C, D
- A, B
- B, C
What is the initial reaction in case of amino acid metabolism?
- Ammonia formation
- Deamination
- Transamination
- Nitrogen formation
Mention the difference(s) between cystine and cysteine.
- Cysteine is found in mammalian cell.
- No disulphide bridge is there in cystine.
- Both 1 and 2
- None of these
State the cause of alkaptonuria.
- Tyrosine hydroxylase
- Deficiency of phenyl alanine hyddroxylase
- Absence of homogentisate oxidase
- Fumarylacetoacetate hydroxylase
Choose the incorrect pair.
- Diabetesis-Hexokinase
- Albinism-Tyrosinase
- Tyrosinosis-Tyrosine hydroxylase
- PKU-Phenylalanine hydroxylase
Which is/are true for electron transport chain?
A. Cytochrome do not participate in electron transfer.
B. Trapping of ATP molecule.
C. Usage of oxygen as final acceptor.
D. Source of electrons for electron transfer in water.
- Only C
- A and D
- B and C
- B, C and D
What is the net yield of complete oxidation of stearic acid?
- 129 ATP
- 146 ATP
- 131 ATP
- 148 ATP
Which of the following statements regarding metabolic pathways is incorrect?
- Many of the steps of glycolysis can run in reverse.
- Starch, sucrose or glycogen must be hydrolysed before it can enter the glycolysis.
- After fats are digested, glycerol enters glycolysis by forming DHAP.
- After fat digestion, fatty acids can no longer participate in cellular respiration.
State the disease caused by sphingomyelinase enzyme.
- Faber’s disease
- Hashimoto disease
- Neimann Pick disease
- Neonatal tyrosinemia
Choose the correct match.
| GROUP I | GROUP II |
| i. Ketolysis | A. Ketone bodies in urine |
| ii. Ketonuria | B. Breakdown of ketone bodies in extrahepatic peripheral tissues |
| iii. Ketonemia | C. Ketone bodies elevation in tissues and blood |
| iv. Ketosis | D. Ketone bodies in blood |
- i - C, ii - A, iii - D, iv - B
- i - B, ii - A, iii - D, iv - C
- i - A, ii - C, iii - D, iv - B
- i - C, ii - A, iii - B, iv - D
Suppose you made an acidic interior of the thylakoids of isolated chloroplasts. Then, you transferred it in the dark to a pH-8 solution.
What would happen?
- Calvin cycle is activated.
- ATPs may be formed from isolated chloroplast.
- Both 1 and 2
- None of these
Suppose you made an acidic interior of the thylakoids of isolated chloroplasts. Then, you transferred it in the dark to a pH-8 solution.
When sunlight is on the chloroplast, what would happen?
- Thylakoids enclose the spaces
- Grana enclose the spaces
- Calvin cycle recurs
- All of the above
A scientist wants to develop a new paddy variety through genetic engineering. This variety has the capacity to withstand cold temperature.
How can the scientist develop this variety?
- Increasing the length of fatty acid chain
- Eliminate all steroids
- Increase frequency of unsaturated fatty acid
- Decrease frequency of unsaturated fatty acid
A scientist wants to develop a new paddy variety through genetic engineering. This variety has the capacity to withstand cold temperature.
What should the scientist do to make that the same plant withstand warm temperature?
- Increase the length of fatty acid chain
- Eliminate all the steroids
- Increase the frequency of unsaturated fatty acid
- Cannot be determined