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Biotechnology

Introduction to fundamental biotechnology concepts, techniques, and tools including genetic engineering, laboratory methods, and molecular biology principles.

10 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

Identify the mismatched pair.

  1. Karl Ereky – coined the term biotechnology
  2. Haberlandt – father of plant tissue culture
  3. G. W. Carver – father of agricultural biotechnology
  4. Wilhelm Johannsen – coined the term gene
  5. G. J. Mendel – father of human genetics
Question 2 Multiple Choice (Single Answer)

Which of the following bacteriophages was the first DNA-based genome to be sequenced?

  1. MS2 phage
  2. Phi X 174 phage
  3. Lambda phage
  4. T4 phage
  5. P1 phage
Question 3 Multiple Choice (Single Answer)

Which of the following bacteriophages infect Bacillus subtilis?

  1. T12 phage
  2. T2 phage
  3. P2 phage
  4. Lambda phage
  5. Phi 29 phage
Question 4 Multiple Choice (Single Answer)

Which of the following statements is INOCRRECT?

  1. The Southern blotting technique depends on similarities between the sequences of probe RNA and experimental RNA.
  2. Electroporation used to transfer polar molecules like DNA into host cells.
  3. The Northern blotting is a laboratory method used to detect specific RNA molecules from a mixture of RNA molecules.
  4. Gel electrophoresis is a basic technique used to separate DNA, RNA or proteins.
  5. PCR is a laboratory technique for amplifying a specific DNA sequence.
Question 5 Multiple Choice (Single Answer)

The term pleiotrophy refers to __________.

  1. combining genes from different species
  2. technique for identification of patterns that occur in DNA
  3. impact of single gene over several traits
  4. inheritance of complex characters that are determined by two or more genes
  5. a form of interaction between nonallelic genes in which one combination of such genes has a dominant effect over other combinations
Question 6 Multiple Choice (Single Answer)

Which of the following enzymes are derived from the fungus Aspergillus niger?

  1. Penicillin amidase, Pectinase, β-glucanase
  2. Lactase, Rennin, Penicillin amidase
  3. Glucoamylase, β-glucanase, Penicillin amidase
  4. Lactase, Rennin, Pectinase
  5. Glucoamylase, Pectinase, β-glucanase
Question 7 Multiple Choice (Single Answer)

Organisms with highest and least number of chromosomes respectively are ___________.

  1. Tympanoctomys barrerae and Myrmecia pilosula
  2. Myrmecia pilosula and Ophioglossum reticulatum
  3. Ophioglossum reticulatum and Myrmecia pilosula
  4. Tympanoctomys barrerae and Drosophila melanogaster
  5. Ophioglossum reticulatum and Tympanoctomys barrerae
Question 8 Multiple Choice (Single Answer)

Which of the following statements is FALSE?

  1. The start codon always codes for methionine in eukaryotes.
  2. The most common start codon is AUG.
  3. The anticodon is a sequence of three base pairs of the tRNA that is complementary to the matching three base pairs on the mRNA.
  4. The termination codon is a nucleotide triplet within tRNA that signals termination of translation.
  5. All are true.
Question 9 Multiple Choice (Single Answer)

Which of following sets represents filamentous bacteriophages only?

  1. T2 phage, f1 phage, fd phage
  2. M13 phage, MS2 phage, f1 phage
  3. M13 phage, T12 phage, fd phage
  4. MS2 phage, T12 phage, f1 phage
  5. M13 phage, f1 phage, fd phage
Question 10 Multiple Choice (Single Answer)

Which of the following statements is not correct?

  1. Plasmids are circular pieces of double stranded DNA that exist separately from chromosomal DNA.
  2. Plasmids that can be propagated in cells of two or more different species are called expression vectors.
  3. Shuttle vectors can be used in both eukaryotes and prokaryotes.
  4. A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted for cloning purposes.
  5. Replacement vector is a cloning vector in which part of the normal genome is replaced by foreign DNA.
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