Nucleic Acid Biochemistry and Molecular Biology Techniques
Quiz covering enzyme functions in nucleic acid metabolism, cDNA synthesis, RNA visualization, hybridization techniques, and protein folding in biochemistry
Questions
Question 1 Multiple Choice (Single Answer)
5' ends of RNA and DNA are dephosphorylated by which of the following enzymes?
- Alkaline phosphatase
- DNase I
- Lambda exonuclease
- DNA ligase
- Terminal transferase
Question 2 Multiple Choice (Single Answer)
Which of the following enzymes is used for removal of hairpin in the synthesis of cDNA?
- Polynucleotide kinase
- S1 nuclease
- DNA polymerase I
- BAL 31 nuclease
- Exonuclease III
Question 3 Multiple Choice (Single Answer)
Which of the following techniques is used for the visualisation of RNA fragments?
- Southern blotting
- Northern blotting
- Southwestern blotting
- Western blotting
- Eastern blotting
Question 4 Multiple Choice (Single Answer)
Which of the following hybridisation techniques is used to identify and purify specific clones?
- DNA-DNA hybridisation
- Fluorescence in-situ hybridisation
- Plaque hybridisation
- Orbital hybridisation
- Nucleic acid hybridisation
Question 5 Multiple Choice (Single Answer)
Which of the following functions is affected in sickle cell anemia?
- Protein folding
- Transcriptional control
- RNA processing
- mRNA production
- Frameshift mutation