Microscopy Methods in Microbiology
Comprehensive quiz covering microscope types, components, and techniques used in microbiology including light, electron, and confocal microscopy
Questions
Who coined the term 'microscope'?
- Galileo Galilei
- Zacharias Janssen
- Robert Hooke
- Giovanni Faber
- Antonie van Leeuwenhoek
The lens found in the eyepiece of a light microsope is called
- diaphragm
- ocular
- nosepiece
- coarse
- condenser
Microscopes are parfocal. What does it mean?
- The image will remain approximately in focus if the magnification changes.
- The image will move from focus if the magnification changes.
- Coarse knob is used to adjust the image.
- Diaphragm is used to adjust the focus.
- The image is seen with a dark background.
The Nobel Prize was awarded to Eric Betzig, William Moerner and Stefan Hell for development in which of the following fields?
- Scanning electron microscopy
- Scanning tunnelling microscopy
- Fluorescence microscopy
- Nomarski microscopy
- Ballistic electron emission microscopy
Which part of a light microscope is used to regulate the amount of passing light?
- Condenser
- Diaphragm
- Fine focus
- Coarse
- Mechanical stage
Which of the following microscopes uses gold coated aluminum stubs for specimen mounting?
- Scanning electron microscope
- Confocal microscope
- Stereoscope microscope
- Transmission electron microscope
- Compound microscope
Which of the following options is an optical technique that increases the sensitivity of standard optical microscope to directly visualise nanometric films?
- CMOS
- SARFAS
- CCD
- Quartz
- SNOM
What should be the respective magnifications for lens settings with objectives of 4x, 10x, 45x and 97x on a microscope with 15x oculars?
- 3.75x, 1.5x, 0.33x and 0.15x
- 0.26x, 0.66x, 3x and 6.46x
- 55x, 145x, 670x and 1450x
- 60x, 150x, 675x and 1455x
- 30x, 75x, 337.5x and 727.5x
Which of the following microscopes uses vaccum medium for microscopy?
- Light microscope
- Confocal microscope
- Stereoscope microscope
- Transmission electron microscope
- Compound microscope
Which of the following microscopes manages focusing by digital computer motorised focusing mechanism?
- Compound microscope
- Stereoscope microscope
- Confocal microscope
- Scanning electron microscope
- Transmission electron microscope
Which of the following microscopes produce(s) 3D images?
- Stereoscope microscope
- Confocal microscope
- Scanning electron microscope
- Transmission electron microscope
- Both (1) and (3)
Match the following:
| (a) Ernst Ruska | (i) Scanning tunnelling microscope |
| (b) Max Knoll | (ii) Atomic force microscope |
| (c) Gerd Binnig and Heinrich Rohrer | (iii) Transmission electron microscope |
| (d) Gerd Binnig, Quate and Gerber | (iv) Scanning electron microscope |
- (a) – (i), (b) – (ii), (c) – (iii), (d) – (iv)
- (a) – (iii), (b) – (ii), (c) – (i), (d) – (iv)
- (a) – (iii), (b) – (iv), (c) – (i), (d) – (ii)
- (a) – (i), (b) – (iii), (c) – (ii), (d) – (iv)
- (a) – (iv), (b) – (iii), (c) – (ii), (d) – (i)
Why is immersion oil put between the slide and the lens in a compound microscope?
- To prevent decrease in resolution
- To prevent increase in resolution
- To enhance decreasing resolution
- To minimise the resolving power of condenser
- None of these
Which of the following is an advantage of in-vivo staining?
- It is used for colouring of cells or structures that have been removed from their biological context.
- It reveals the locations where certain chemical reactions are taking place.
- Techniques like fixation can be used as diagnostic tools.
- Techniques like sampling can be used repeatedly.
- It visualises fixed cells.
Which of the following microscopes uses laser light for providing specimen contrast?
- Stereoscope microscope
- Confocal microscope
- Scanning electron microscope
- Transmission electron microscope
- Compound microscope