Biochemistry and Biotechnology
Covers fundamental biochemistry topics including amino acids, proteins, polysaccharides, single cell protein, electrophoresis, coenzymes, immunoglobulins, and antibiotic mechanisms
Questions
Which of the following causes food poisoning?
- Clostridium perfringens
- Streptococcus pyrogenes
- Escherichia coli
- Yersinia pestis
- Mycobcterium bovis
Threonine amino acid is biotechnologically produced by
- bacillus subtilis
- mutants of Ecoli
- brevibacterium
- corynebacterium glutamicum
- pseudomonas
Which media cannot be used in electrophoresis?
- Cellulose acetate
- Paper
- Agar gel
- Starch grains
- Polyacrylamide gel
Which of the following statements is false about single cell protein?
- Single cell protein refers to the mass culture of certain algae, bacteria, yeasts, moulds etc.
- Scp is also produced using carbohydrates as raw materials.
- SCP is also produced via paper industry waste.
- Spirullina a cyanobacterium offers excellent prospects for low technology SCP production.
- Paraffins and sulphide liquor cannot be used as raw materials for the production of SCP.
Which protein is not protective in function?
- Antibodies
- Fibrinogen
- Thrombin
- Serum albumin
- None of the above
Which is the most abundant polysaccharide found in skin and cartilaginous tissue?
- Heparin
- Hyaluronic acid
- Chondrotin sulfate
- Murein
- Capsules
Where are B lymphocytes located?
- Efferent lymph
- Blood and lymphoid tissue
- Blood
- Lymph nodes
- Reticuloendothelial cells
Live attenuated organisms as vaccine are used in case of
- tuberculosis
- tetanus
- diphtheria
- cholera
- polio
Which of the following statements is false regarding the limitations of Beer-Lambert law?
- Light must be in narrow wavelength range.
- The wavelength of light used should be the absorption maximum of the solution.
- The law only holds up to a threshold maximum concentration for a given substance.
- There must be no ionisation, association or dissociation of the solute with concentration or time.
- This law has no limitation.
Which of the following coenzymes is not involved in group transfer reactions?
- TPP
- Coenzyme A
- Tetrahydrofolic acid
- S-adenosyl methionine
- Ferro porphyrins
Which immunoglobulin is found abundantly in internal body fluid?
- IgA
- IgD
- IgE
- IgM
- IgG
What is the mode of action of cycloserine antibiotic?
- Interference with regeneration of the monophosphate form of bactoprenol from the pyrophosphate form
- Inhibition of peptidoglycan synthesis
- Damage of membrane
- Inhibits peptidoglycan synthesis by interference with synthesis of the peptide moiety of the peptidoglycan
- Causing a leakage from the cytoplasmic content of the cell
Tetracycline acts by which of the following?
- Inhibiting protein synthesis through interference with the binding of aminoacyl t RNA to the 30 S subunit ribosome.
- Inhibiting protein synthesis by combining with the 50S subunit ribosome.
- Inhibiting protein synthesis by combining irreversibly with the 30 S subunit.
- Increases cell permeability.
- Causing a leakage from the cytoplasmic content of the cell.