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Genetic Engineering and Biotechnology

A comprehensive quiz covering genetic engineering techniques, molecular biology tools, vectors, restriction enzymes, transgenic organisms, and applications of biotechnology in agriculture and medicine.

25 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

The vector for transfer of DNA to plants is

  1. T1 plasmid
  2. pBR322
  3. YAC
  4. M13
Question 2 Multiple Choice (Single Answer)

Golden rice is rich in

  1. vitamin A
  2. vitamin B
  3. vitamin C
  4. vitamin D
Question 3 Multiple Choice (Single Answer)

The father of genetic engineering is

  1. Cohen and Boyer (1973)
  2. Arber, Nathan and Smith(1970)
  3. Paul Berg (1972)
  4. Boliver and Rodriguez
Question 4 Multiple Choice (Single Answer)

Restriction enzymes are known as

  1. Molecular blades
  2. Molecular scissors
  3. Molecular cutters
  4. Vectors
Question 5 Multiple Choice (Single Answer)

Which of the following is responsible for the initiation of replication?

  1. Origin of replication
  2. Plasmid
  3. Restriction endonuclease
  4. Vectors
Question 6 Multiple Choice (Single Answer)

The function of exonuclease is

  1. to remove nucleotides from the ends of DNA
  2. to add nucleotides to the ends of DNA
  3. to remove nucleotides at specific position
  4. to add nucleotides at specific position
Question 7 Multiple Choice (Single Answer)

Pallindromes are

  1. base pair sequences which are same when read forward and backward
  2. different base pair sequences when read in forward and backward
  3. made of same base pair
  4. not recognised by restriction endonuclease
Question 8 Multiple Choice (Single Answer)

Key tools of genetic engineering are all except

  1. Restriction enzymes
  2. Vectors
  3. Polymerase enzymes
  4. Helicase enzymes
Question 9 Multiple Choice (Single Answer)

The function of DNA LIGASE is

  1. to unwind DNA
  2. to make cuts at specific positions
  3. to form a phosphodiester bond between 5' phosphate group of one nucleotide and 3' hydroxyl group of another nucleotide.
  4. to form a phosphodiester bond between 3' phosphate group of one nucleotide and 5' hydroxyl of other nucleotide.
Question 10 Multiple Choice (Single Answer)

Alkaline phosphatase has a role in

  1. removing of unwanted nucleotides from the end of DNA
  2. adding of more nucleotides to DNA
  3. the removal of phosphate group
  4. adding of methyl group to one end of DNA
Question 11 Multiple Choice (Single Answer)

Which of the following restriction enzymes is used in genetic engineering?

  1. Type one
  2. Type two
  3. Type three
  4. Type four
Question 12 Multiple Choice (Single Answer)

Which enzyme can withstand high temperature (>90 c)?

  1. Thermus aquaticus
  2. Taq polymerase
  3. Cellulase
  4. Lysozyme
Question 13 Multiple Choice (Single Answer)

The dye used for staining DNA fragments in electrophoresis is

  1. Ethidium bromide
  2. Ethidium chloride
  3. Ethidium flouride
  4. Ethidium iodide
Question 14 Multiple Choice (Single Answer)

Bt cotton is concerned with

  1. herbicide intolerance
  2. low yield
  3. pest resistance
  4. plant height
Question 15 Multiple Choice (Single Answer)

Colchine and Granosan are involved in

  1. inhibition of chromosome replication
  2. increases cell division
  3. prevents the spindle formation
  4. inhibits metaphase
Question 16 Multiple Choice (Single Answer)

Brazzein is

  1. an enzyme
  2. a low calorie sweetener
  3. cholestrol reducing
  4. produced by animal
Question 17 Multiple Choice (Single Answer)

What is the function of Hirudin?

  1. It prevents blood clotting.
  2. It prevents heart attack.
  3. It is produced by bacteria.
  4. It prevents kidney faliure.
Question 18 Multiple Choice (Single Answer)

Molecular glue is another name for

  1. DNA Ligase
  2. restriction enzyme
  3. DNA polymerase
  4. Topoisomerase
Question 19 Multiple Choice (Single Answer)

Transfer of genetic material from one bacterium to another by agency of viruses is called

  1. Transduction
  2. Conjugation
  3. Transformation
  4. Hybridisation
Question 20 Multiple Choice (Single Answer)

Which of the following statements is correct about plasmids?

  1. They are required by bacteria
  2. They help in reproduction
  3. They are small bacteria
  4. They are self replicating, xtrachromosomal DNA fragnents
Question 21 Multiple Choice (Single Answer)

Chemical knives of DNA are

  1. DNA polymerase
  2. Restriction enzyme
  3. RNA synthetase
  4. Reverse transcriptase
Question 22 Multiple Choice (Single Answer)

Electrophoresis means

  1. separation on the basis of charge
  2. separation on the basis of mass
  3. separation on the basis of density
  4. separation on the basis of volume
Question 23 Multiple Choice (Single Answer)

The process of making temporary opening in cell membrane to introduce foreign DNA is

  1. microinjection
  2. particle gun method
  3. electroporation
  4. chemical mediated genetic transformation
Question 24 Multiple Choice (Single Answer)

DNA polymerase was discovered by

  1. A.Korenberg
  2. Paul Berg
  3. A.Tiselius
  4. Cohen & Boyer
Question 25 Multiple Choice (Single Answer)

Flavr Savr is transgenic variety of

  1. tomato
  2. rice
  3. cotton
  4. wheat
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