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Microbiology

Methods and Techniques in microbiology Methods in Microbiology

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Question 1 Multiple Choice (Single Answer)

Eosin Methylene Blue is a stain for Gram negative bacteria. It contains Eosin and Methylene blue in the ratio of

  1. 3 : 1
  2. 1 : 3
  3. 6 : 1
  4. 1 : 6
  5. 1 : 1
Question 2 Multiple Choice (Single Answer)

Which of the following is not an acid fast structure?

  1. Oocysts of Cryptosporidium parvum
  2. Actinomycetes
  3. Bacterial spores
  4. Streptomyces
  5. Mycobacteria
Question 3 Multiple Choice (Single Answer)

Traditional stain used for mitochondria is

  1. crystal violet
  2. acid fuchsine
  3. haematoxylin
  4. cresyl violet
  5. nile blue
Question 4 Multiple Choice (Single Answer)

What is Indian ink?

  1. Negative stain
  2. Acid fast
  3. Gram stain
  4. Endosopore stain
  5. Romanowsky stain
Question 5 Multiple Choice (Single Answer)

Firmacutes (+), Gracillicutes (−), Mollicutes (0) and Mendocutes (var.) are four divisions of the kingdom Monera, divided on the basis of

  1. endospore staining
  2. simple staining
  3. Albert’s staining
  4. acid fast staining
  5. Gram staining
Question 6 Multiple Choice (Single Answer)

Which of the following is a negatively charged stain (anion)?

  1. Methyle blue
  2. Crystal violet
  3. Hemotoxylene
  4. Eosin
  5. Basic fuchsin
Question 7 Multiple Choice (Single Answer)

Which of the following is a basic dye?

  1. Aniline blue
  2. Crystal violet
  3. Orange G
  4. Eosin
  5. Fast green
Question 8 Multiple Choice (Single Answer)

Pap staining is a frequently used method for examining cell samples from various bodily secretions. Which of the following combinations does it use?

  1. Haematoxylin, Crytal violet, Orange G, eosin Y, Light Green SF yellowish and sometimes Bismarck Brown Y
  2. Haematoxylin, Orange G, eosin Y, Light Green SF yellowish and sometimes Bismarck Brown Y
  3. Haematoxylin, Orange G and sometimes Bismarck Brown Y
  4. Haematoxylin, Crytal violet, Orange G, eosin Y, Light Green SF yellowish
  5. Light Green SF yellowish, iodine, methylene blue and sometimes Bismarck Brown Y
Question 9 Multiple Choice (Single Answer)

Match the following columns:
(a) Tissues that stain by acidic dyes (i) Chromophobic
(b) Tissues that stain by basic dyes (ii) Acidophilic
(c) Tissues that do not take up coloured dye readily (iii) Amphophilic
(d) Tissues that stain with either acid or basic dyes (iv) Basophilic
  1. (a) – (iv), (b) – (ii), (c) – (iii), (d) - (i)
  2. (a) – (ii), (b) – (i), (c) – (iv), (d) - (iii)
  3. (a) – (ii), (b) – (iv), (c) – (i), (d) - (iii)
  4. (a) – (iv), (b) – (iii), (c) – (i), (d) - (ii)
  5. (a) – (i), (b) – (iv), (c) – (ii), (d) - (iii)
Question 10 Multiple Choice (Single Answer)

Which of the following statements is false?

  1. Gram staining was developed by Dutch Physician Hans Christian Gram in 1883.
  2. Gram positive bacteria has a thick peptidoglycan layer.
  3. Gram negative bacteria has a very low lipid content in cell wall.
  4. In Gram staining, alcohol acts on membrane lipids.
  5. Acid fast stain was developed by Franz Ziehl and the pathologist Friedric Neelsen.
Question 11 Multiple Choice (Single Answer)

Match the following columns:

 
(a) Eschericia coli (i) Gram positive
(b) Mycobacterium phei (ii) Gram negative
(c) Staphylococcus epidermi (iii) Waxy/crumby gram positive rods
(d) Mycobacterium smegmatis (iv) Opportunistic gram positive acid-fast
  1. (a) – (iv), (b) – (ii), (c) – (iii), (d) - (i)
  2. (a) – (ii), (b) – (i), (c) – (iv), (d) - (iii)
  3. (a) – (ii), (b) – (iv), (c) – (i), (d) - (iii)
  4. (a) – (iv), (b) – (iii), (c) – (i), (d) - (ii)
  5. (a) – (i), (b) – (iv), (c) – (ii), (d) - (iii)
Question 12 Multiple Choice (Single Answer)

The outer membrane of gram-negative bacteria contains

  1. cytoplasm
  2. teichoic acids
  3. a thick peptidoglycan layer
  4. lipoteichoic acids
  5. lipopolysaccharide
Question 13 Multiple Choice (Single Answer)

Which of the following stain(s) is/are specially used for cell cycle determination and gel electrophosis?

  1. Acridine orange
  2. Malachite green
  3. Ethidium bromide
  4. Acridine orange and Ethidium bromide
  5. Phosphotungstic acid
Question 14 Multiple Choice (Single Answer)

Which of the following options has Acid Fast organisms?

  1. Staphylococcus, Streptococcus, Clostridium and Nocardia
  2. Cryptospordium parvum, isospora, cyclospora cysts
  3. Enterobacteriaceae, vibrio cholera, pseudomonas aeruginosa, mycobacterium leprae
  4. Legionella micdadei, nocardia, head of sperm
  5. Both (2) and (4)
Question 15 Multiple Choice (Single Answer)

Match the following columns:

 
(a) Limit of resolution for Human Eye (i) 0.2m or 2000Ao
(b) Limit of resolution for Light Microscope (ii) 0.5nm or 5Ao
(c) Limit of resolution for Scanning Electron Microscope (iii) 0.1mm or 100um
(d) Limit of resolution for Transmission Electron Microscope (iv) 5 - 10nm or 50-100 Ao
  1. (a) – (iii), (b) – (i), (c) – (ii), (d) – (iv)
  2. (a) – (ii), (b) – (i), (c) – (iv), (d) – (iii)
  3. (a) – (iii), (b) – (i), (c) – (iv), (d) – (ii)
  4. (a) – (i), (b) – (iii), (c) – (iv), (d) – (ii)
  5. (a) – (iv), (b) – (i), (c) – (iii), (d) – (ii)
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