Biotechnology (GATE)
Comprehensive biotechnology quiz covering biochemistry, microbiology, process biotechnology, molecular biology, genetics, biomolecules, enzymes, and recombinant DNA technology.
Questions
Which of the following proteins have the least number of polypeptide chains?
- Chymotrypsin
- Haemoglobin
- Hexokinase
- Glutamine synthetase
- Ribonuclease A
__________________ are enzymes which cleave DNAs at specific base sequences.
- Type II restriction endonucleases
- Polynucleotide kinase
- Terminal transferase
- Exonuclease III
- Bacteriophage λ exonuclease
Choose the option that shows the correct pairing of the diseases with their corresponding causative organisms.
| P. Chagas disease | i. Trypanosoma gambiense |
| Q. Sleeping sickness | ii. Plasmodium falciparum |
| R. Malaria | iii. Yersinia pestis |
| S. Plague | iv. Trypanosoma cruzi |
- P - i, Q - iv, R - iii, S - ii
- P - i, Q - iv, R - ii, S - iii
- P - ii, Q - iv, R - iii, S - i
- P - iv, Q - i, R - ii, S - iii
- P - iv, Q - i, R - iii, S - ii
Which of the following is a steroid hormone?
- Insulin
- Glucagon
- Epinephrine
- Testosterone
- Triiodothyronine
___________________ utilises tetrahydrofolate as a prosthetic group.
- Pyruvate dehydrogenase
- Monoamine oxidase
- Acetyl CoA carboxylase
- Thymidylate synthase
- Methylmalonyl mutase
Choose the option that shows the correct pairing of the products with their corresponding microorganisms.
| P. Citric acid | i. Micromonospora purpurea |
| Q. Polyhydroxyalkonates | ii. Zymomonas mobilis |
| R. Gentamycin | iii. Aspergillus niger |
| S. Ethanol | iv. Ralstonia eutropha |
- P - i, Q - ii, R - iii, S - iv
- P - ii, Q - iii, R - i, S - iv
- P - iii, Q - iv, R - i, S - ii
- P - iii, Q - i, R - iv, S - ii
- P - iv, Q - ii, R - iii, S - i
Match the hemoproteins in column I with their function in column II.
| Column I |
</strong>|<strong>Column II</strong>|
|P. Haemoglobin|1. Degradation of hydrogen peroxide|
|Q. Myoglobin|2. Carrying of oxygen molecules to muscle tissues|
|R. Cytochromec|3. Transport of oxygen in blood|
|S. Catalase|4. Involvement in electron transport chain|
- P - 1; Q - 2; R - 3; S - 4
- P - 2; Q - 1; R - 4; S - 3
- P - 3; Q - 2; R - 4; S - 1
- P - 3; Q - 4; R - 1; S - 2
- P - 4; Q - 3; R - 2; S - 1
Arrange the following organisms in increasing order of genome size.
- Borrelia burgdorferi
2. Helicobacter pylori
3. Mycoplasma pneumoniae
4. Treponema pallidum
- 1 < 2 < 3 < 4
- 1 < 3 < 2 < 4
- 2 < 4 < 1 < 3
- 3 < 4 < 1 < 2
- 3 < 1 < 4 < 2
Cyanogen bromide catalyses the hydrolysis of only those peptide bonds in which
- the carbonyl group is contributed by Lys or Arg residue
- the carbonyl group is contributed by Phe, Trp or Tyr residue
- the carbonyl group is contributed by Met residue
- the amino group is contributed by Phe, Trp or Tyr residue
- All of the above
Which of the following enzymes is used in recombinant DNA technology for making a DNA copy of an RNA molecule?
- DNA ligase
- DNA polymerase I
- Reverse transcriptase
- Terminal transferase
- Alkaline phosphatase
Which of the following is a set of heteropolysaccharides?
- Cellulose, Chitin, Dextran
- Cellulose, Dextran, Agarose
- Peptidoglycan, Agarose, Chitin
- Peptidoglycan, Agarose, Hyaluronate
- Peptidoglycan, Hyaluronate, Dextran
Which of the following is an example of lyases?
- Lactate dehydrogenase
- Nucleoside monophosphate kinase
- Fumarase
- Triose phosphate isomerase
- Chymotrypsin
Zn2+ serves as a cofactor for
- arginase
- hexokinase
- carbonic anhydrase
- glutathione peroxidise
- pyruvate kinase
Which of the following 'disease: causative agent' matches is INCORRECT?
- Tuberculosis: Mycobacterium
- Tetanus: Clostridium
- Botulinum: Treponema
- Bacillary dysentery: Shigella
- Cholera: Vibrio
Match enzymes in column I with respective coenzymes in column II.
| Column I |
</strong>|<strong>Column II</strong>|
|P. Pyruvate carboxylase|1. Biotin|
|Q. Pyruvate dehydrogenase|2. Thiamine pyrophosphate|
|R. Glycogen phosphorylase|3. Pyridoxal phosphate|
|S. Lactate dehydrogenase|4. Nicotinamide adenine dinucleotide|
- P - 1; Q - 2; R - 3; S - 4
- P - 1; Q - 3; R - 2; S - 4
- P - 2; Q - 1; R - 3; S - 4
- P - 2; Q - 1; R - 4; S - 3
- P - 3; Q - 2; R - 4; S - 1