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Molecular Biology and Biotechnology Techniques

Quiz covering fundamental concepts in molecular biology, DNA structure, recombinant DNA technology, and laboratory techniques used in genetic research

8 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

Which one of the following ‘Year: Event’ pairs is not correct?

  1. 1956 - DNA is made artificially
  2. 1969 - The first single gene is isolated
  3. 1970 - The first artificial gene
  4. 1983 - The first artificial chromosome
  5. 1998 – First draft of human genome is published
Question 2 Multiple Choice (Single Answer)

The first DNA-based genome sequence is ______

  1. Lambda phage
  2. P1 phage
  3. T4 phage
  4. MS2 phage
  5. phi X 174 phage
Question 3 Multiple Choice (Single Answer)

Which among the following statements is/are not correct?
1. The start codon always codes for methionine in eukaryotes.
2. The most common start codon is AUG.
3. The anticodon is a sequence of three base pairs of the tRNA that is complementary to the matching three base pairs on the mRNA.
4. The termination codon is a nucleotide triplet within tRNA that signals the termination of translation.

  1. Only 1
  2. Only 2
  3. 1 and 2
  4. 1, 2 and 3
  5. Only 4
Question 4 Multiple Choice (Single Answer)

Identify the incorrect statement/s.

  1. A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted for cloning purposes.
  2. Shuttle vectors can be used in both eukaryotes and prokaryotes.
  3. Plasmids that can be propagated in cells of two or more different species are called expression vectors.
  4. Plasmids are circular pieces of double stranded DNA that exist separately from chromosomal DNA.
  5. Replacement vector is a cloning vector in which part of the normal genome is replaced by foreign DNA.
Question 5 Multiple Choice (Single Answer)

Which among the following statements is/are not true?

  1. The Northern blotting is a laboratory method used to detect specific RNA molecules from a mixture of RNA molecules.
  2. PCR is a laboratory technique for amplifying a specific DNA sequence.
  3. Electroporation used to transfer polar molecules like DNA into host cells.
  4. The Southern blotting technique depends on similarities between the sequences of probe RNA and experimental RNA.
  5. Gel electrophoresis is a basic technique used to separate DNA, RNA or proteins.
Question 6 Multiple Choice (Single Answer)

Choose the correct statement.

  1. 11 base pairs are present in one turn of DNA.
  2. Anticodon occurs in transfer-RNA.
  3. Adenine is an amino acid.
  4. Distance between two strands of DNA is 15 Å.
  5. Distance between two base pairs of DNA is 34 nm.
Question 7 Multiple Choice (Single Answer)

Identify the incorrectly matched pair?

  1. Adenosine: Adenine and ribose sugar
  2. Guanidine: Guanine and ribose sugar
  3. Nucleotide: Nitrogen base, sugar and phosphate
  4. Nucleoside: Pentose sugar and nitrogen base
  5. Both 2 and 4
Question 8 Multiple Choice (Single Answer)

Match the column-I with column-I
Column I 
        </strong>|<strong>Column II</strong>|

|1. Recombinant DNA technology|a. Technique for identification of patterns that occur in DNA|
|2. Restriction fragments|b. All the genes contained in one kind of organism|
|3. Genomic library|c. Pieces of DNA that have been cut into different lengths|
|4. Restriction enzymes|d. DNA cutting enzymes|
|5. DNA fingerprinting|e. Combining genes from different species|
| |f. A collection of cloned DNA pieces from a genome.|

  1. 1 - e, 2 - c, 3 - f, 4 - d, 5 - a
  2. 1 - a, 2 - c, 3 - b, 4 - d, 5 - e
  3. 1 - f, 2 - d, 3 - a, 4 - c, 5 - b
  4. 1 - a, 2 - b, 3 - d, 4 - a, 5 - f
  5. 1 - b, 2 - c, 3 - a, 4 - d, 5 - e
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