Biotechnology and Its Applications (Class XII)
Biology-Biotechnology
Questions
HaeIII breaks DNA at 5'-GGCC-3'. Which fragments are given by it?
- Sticky end fragments
- Smooth end fragments
- Rough end fragments
- Blunt end fragments
- Gum end fragments
Which of the following is the correct explanation of Gibson assembly?
- Joining DNA fragment with RNA fragment by ligase
- DNA fragments combining by restriction enzyme/ligase cloning
- Binding RNA fragments
- Plasmid assembly
- Joining rRNA with mRNA
Which important parts are present in pBR322?
- Penicillin--Ori--EcoRI; HindIII, EcoRV, BamHI
- Lipramycin--EcoRI; HindIII, EcoRV, BamHI
- Cephalosporin--Ori--EcoRI; HindIII, EcoRV, BamHI
- amp--tet--Ori--EcoRI; HindIII, EcoRV, BamHI
- Ampicillin--terramycin--Ori--EcoRI; HindIII, EcoRV, BamHI
What is the main purpose of rDNA technology?
- DNA cloning
- DNA-RNA hybrid cloning
- RNA cloning
- To study transformation
- To study gene mutation
Which DNA is developed from RNA precursor?
- rDNA
- mDNA
- Naked DNA
- ssDNA
- cDNA
Which of the following is called hybridisation probe?
- Any heavy ds/ss DNA fragment
- Any heavy ds/ss RNA fragment
- Radioactively labelled DNA/RNA fragment
- Heavy element labelled lipid fragment
- Heavy isotope labelled protein
Where is rDNA multiplied?
- In target vector
- In cloning vector
- In viruses
- In archaebacteria
- In eukaryotic protista
Which chemical is used for transformation of rDNA into cloning vector?
- Calcium phosphate
- Calcium chloride
- Sodium chloride
- Sodium alginate
- Glycol
Which of the following colour marker chemicals is mixed to the medium to identify rDNA cloned vectors?
- x-gal
- Calcium alginate
- Thymol blue
- Safranine
- Phenophthalene
Which of the following correctly describes rDNA?
- Crossover DNA
- Mutated DNA
- Hybrid DNA
- Transcripted DNA
- Plasmid DNA
Which of the following is the correct use of thermocycler?
- To heat the bacterial medium
- For sterile inoculation
- Amplification of DNA fragment
- For autoclaving
- For hybridisation
Which characters should be possessed by a plasmid for rDNA technology?
- Genes related to ori-restriction site
- Genes of ori - insulin gene - restriction site
- Colicin gene - ori - restriction site
- Cloning gene - colicin gene - ori
- Ori - colicin gene - insulin gene
Restriction endonucleases break dsDNA at ______ in _______ direction.
- episome, 5'-3'
- episome, 3'-5'
- palindrome, 5'-3'
- palindrome, 3'-5'
- perisome, 5'-3'
Which type of endonucleases are generally used in genetic engineering?
- Type-I
- Type-II
- Type-III
- Type-IV
- Type-V
Which is the recognition site of EcoR I?
- 5'--GAT ATC--3'
- 5'--- CCWGG--3'
- 5'--G AATTC--3'
- 5'--G GATCC--3'
- 5'----A AGCTT---3'