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Which of the given structures has a role of permeability barrier in preventing the loss of the primary stain crystal violet in gram staining procedure?

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A
Peptidoglycan
💡 Explanation:

Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet. Gram negative bacteria has a thinner peptidoglycan wall, which does not retain the crystal violet during the decolouring process.

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