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Methods in Microbiology

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Which of the following are used as counterstain in Gram staining technique?
P. Crystal violet
Q. Safranin
R. Iodine solution
S. Carbol fuchsin
Select the correct answer.

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A
Q and S
💡 Explanation:

Safranin is used as secondary stain (counterstain) in Gram staining technique.
Carbol fuchsin is sometimes substituted for safranin as counterstain, since it  more intensely stains anaerobic bacteria.

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