Recombinant DNA (GATE)
Questions related to recombinant DNA technology, vectors, restriction enzymes, PCR, and genetic engineering applications for GATE preparation.
Questions
Which of the following pairs is incorrect?
- Biopiracy : Exploitation of bioresources without authentic permission
- Bioethics : Moral guidance to the problems in biology
- Biopotency : Measure of the ability of a material to have a specified biochemical function
- Cryopreservation : Preserving germplasm in frozen state
- Biolistics : Technique of using very small metal particles coated with desired gene in gene transfer
Which of the following statements is incorrect?
- Southern blotting technique depends on similarities between the sequences of probe RNA and experimental RNA.
- Electroporation is used to transfer polar molecules like DNA into host cells.
- Northern blotting is a laboratory method used to detect specific RNA molecules from a mixture of RNA molecules.
- Gel electrophoresis is a basic technique used to separate DNA, RNA or proteins.
- PCR is a laboratory technique for amplifying a specific DNA sequence.
Which of the following statements is false?
- The term enzyme was proposed by Kuhne.
- The first enzyme molecule to be isolated in pure crystalline form was urease.
- Synthesis of enzymes is regulated by inducer and repressor genes.
- DNA ligase was first isolated from bacteriophages.
- Ribozyme is an RNA with enzyme activity.
Directions: Read the given statements and choose the correct option.
Assertion: Flavr Savr tomato was the first commercially grown genetically engineered food to be granted a license for human consumption.
Reason: Flavr Savr tomatoes were developed using recombinant DNA techniques to express the trait of early softening of tomato fruit.
- A and R are both correct and R is the correct explanation of A.
- A and R are both correct and R is not the correct explanation of A.
- A is correct and R is incorrect.
- A is incorrect and R is correct.
- A and R are both incorrect.
Which of the following statements is not correct?
- Plasmids are circular pieces of double-stranded DNA that exist separately from chromosomal DNA.
- Plasmids that can be propagated in cells of two or more different species are called expression vectors.
- Shuttle vectors can be used in both eukaryotes and prokaryotes.
- A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted for cloning purposes.
- Replacement vector is a cloning vector in which part of the normal genome is replaced by foreign DNA.
Match the following:
| Column – I | Column – II |
| P. Erythropoietin | 1. Lymphoblastic leukaemia |
| Q. Factor VIII | 2. Haemophilia |
| R. Pegaspargase | 3. Anaemia |
| S. Superoxide dismutase | 4. Heart attack |
- P - 1, Q - 2, R - 3, S - 4
- P - 2, Q - 1, R - 4, S - 3
- P - 3, Q - 2, R - 1, S - 4
- P - 4, Q - 3, R - 2, S - 1
- P - 1, Q - 2, R - 4, S - 3
Which of the following matchings is not correct?
- Recombinant DNA technology - combining genes from different species
- Restriction fragments - pieces of DNA that have been cut into different lengths
- Genomic library - all the genes contained in one kind of organism
- Restriction enzymes – DNA-cutting enzymes
- DNA fingerprinting - technique for identification of patterns that occur in DNA
Directions: Read the given statements and choose the correct option.
Assertion: Zinc-finger nucleases (ZFNs) are artificial restriction enzymes.
Reason: The DNA-binding domains of individual ZFNs typically contain between nine and eighteen individual zinc finger repeats.
- A and R are both correct and R is the correct explanation of A.
- A and R are both correct and R is not the correct explanation of A.
- A is correct and R is incorrect.
- A is incorrect and R is correct.
- A and R are both incorrect.