Multiple choice What was the root cause of the Peloponnesian War? Alexander the Great was going to take over the Greek Empire The Christians were being persecuted There was conflict between the Spartans and Athenians The emperor was burning books Reveal answer Fill a bubble to check yourself C Correct answer Explanation The Peloponnesian War was a major conflict in ancient Greece fought between the Delian League (led by Athens) and the Peloponnesian League (led by Sparta).