Multiple choice Fructose 6-phosphate forms Fructose 1,6-Bisphosphate in presence of enzyme PFK-1 PFK-2 Both None Reveal answer Fill a bubble to check yourself A Correct answer Explanation Phosphofructokinase-1 (PFK-1) is the primary enzyme responsible for the phosphorylation of Fructose 6-phosphate to Fructose 1,6-bisphosphate in glycolysis.