Multiple choice DNA Polymerase III can only add new nucleotides to the ? of the newly growing strand. 5 prime end 3 prime end 4 prime end 2 prime end Reveal answer Fill a bubble to check yourself B Correct answer Explanation DNA polymerase III requires a free 3' hydroxyl group to attach a new nucleotide. Therefore, it can only extend a DNA strand in the 5' to 3' direction, adding to the 3' end.