Multiple choice What is the role of DNA Polymerase I? To replace the DNA primer with RNA nucleotides To join together the Okazaki fragments on the leading strand To join together the Okazaki fragments on the lagging strand To replace the RNA primer with DNA nucleotides Reveal answer Fill a bubble to check yourself D Correct answer Explanation DNA Polymerase I is specifically responsible for removing the RNA primers used during replication and replacing them with the appropriate DNA nucleotides. DNA Polymerase III is the primary enzyme for elongation.