Multiple choice After presynaptic neurons are depolarized they: Release a neurotransmitter into the synapse Trigger an action potential within the synapse Produce acetylcholine in the cytoplasm Stimulate hyperpolarization Reveal answer Fill a bubble to check yourself A Correct answer Explanation When the presynaptic membrane depolarizes, voltage-gated calcium channels open, leading to an influx of calcium which triggers the exocytosis of neurotransmitters into the synaptic cleft.