Multiple choice Acetylcholine (ACh) is broken down in the synapse into: Choline and acetate by acetyl coenzyme A Choline and acetate by acetylcholinesterase Choline and acetyl coenzyme A by acetase Choline and acetyl coenzyme A by acetylcholinesterase Reveal answer Fill a bubble to check yourself B Correct answer Explanation Acetylcholinesterase is the specific enzyme located in the synaptic cleft that hydrolyzes acetylcholine into choline and acetate.