Which of the following vector is obtained by the deletion of the poison sequence of pBR322?

pBR322 has nic-bom region which is responsible for its mobilization.Mobilization refers to the cell to cell transfer of an otherwise conjugative plasmid inside the same cell as former. The nic-bom region also interferes with replication efficiency of extrachromosomal DNA in monkey cells,such prokaryotic sequence are called poison sequences. The poison sequence of pBR322 has been deleted to yield pBR327 .