Multiple choice

The concentration of nucleotides and nucleic acids are often expressed in terms of ________.

  1. absorbance at 260 nm

  2. absorbance at 360 nm

  3. absorbance at 450 nm

  4. absorbance at 110 nm

  5. absorbance at 540 nm
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A Correct answer
Explanation

At pH 7.0 all common nucleotides absorb light at a wavelength close to 260 nm. Conjugated double bonds of purines and pyrimidine derivatives absorb ultravoilet light.

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