Multiple choice

Which of the following sequencing methods is a non-enzymatic method of DNA sequencing?

  1. Microscopy-based techniques

  2. Sequencing by hybridization

  3. Tunneling currents DNA sequencing

  4. RNAP sequencing

  5. Microfluidic Sanger sequencing
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Sequencing by hybridization is a non-enzymatic method that uses a DNA microarray. A single pool of DNA whose sequence is to be determined, is fluorescently labeled and hybridized to an array containing known sequences. Strong hybridization signals from a given spot on the array identify its sequence in the DNA being sequenced.

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