Multiple choice

Genetic engineering is the process of transferring specific genes from the chromosome of one organism and transplanting them into the chromosome of another organism. Which among the following statements concerning the process of genetic engineering is false?

  1. The desired DNA is cleaved from the donating chromosome by the action of restriction enzymes.

  2. The desired DNA fragment is inserted into a vector, usually a viral particle, for transfer to the receiving chromosome.

  3. When the host cell reproduces, the vectors inside also reproduce.

  4. Gel electrophoresis is used to separate the remaining genes by movement on an electric grid.

  5. Radioactive probes are added in the gel electrophoresis which attract and bind with the desired DNA to produce visible bands.
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B Correct answer
Explanation

The desired DNA fragment is inserted into a vector, usually a plasmid, for transfer to the receiving chromosome. Plasmids are circular DNA molecules found in the cytoplasm of bacteria that bond with the desired DNA fragment with the help of the joining enzyme, DNA ligase, to create the resulting recombinant DNA.

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