Multiple choice

Which of the following technique is used to detect methylation of DNA?

  1. Western blotting

  2. Souther blotting

  3. Northern blotting

  4. Bisulfide blotting

  5. ELISA

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected.Thus, bisulfite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues.