Multiple choice A nerve fibre is depolarized when it acquires a negative charge in the intracellular fluid and positive charge outside a positive charge in the intracellular fluid and negative charge outside positive charge on both sides negative charge on both sides Reveal answer Fill a bubble to check yourself B Correct answer Explanation Depolarization occurs when the membrane potential becomes less negative (or more positive) inside the cell relative to the outside, typically due to an influx of sodium ions.