Multiple choice

Which of following techniques does not require dyes and stain to produce a coloured effect in microscopy?

  1. Dispersion staining

  2. Phosphotungstic acid

  3. Osmium tetroxide

  4. Neutral red

  5. Conklin's staining

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A Correct answer
Explanation

Dispersion staining is an optical technique that results in a coloured image of a colourless object. This is an optical staining technique and requires no stains or dyes to produce a coloured effect. There are five different microscope configurations used in the broader technique of dispersion staining. They include brightfield Becke line, oblique, darkfield, phase contrast, and objective stop dispersion staining.