Multiple choice Which of the following inhibits myosin-actin binding in relaxed muscles of the body? Troponin T Troponin C EF hand Troponin I Calmodulin Reveal answer Fill a bubble to check yourself D Correct answer Explanation Troponin binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle.