Multiple choice The function of DNA LIGASE is to unwind DNA to make cuts at specific positions to form a phosphodiester bond between 5' phosphate group of one nucleotide and 3' hydroxyl group of another nucleotide. to form a phosphodiester bond between 3' phosphate group of one nucleotide and 5' hydroxyl of other nucleotide. Reveal answer Fill a bubble to check yourself C Correct answer Explanation Correct.