Multiple choice

The usual steam temperature in an autoclave for sterilisation of culture media 15 lbs/inch2 is

  1. 100oC

  2. 98oC

  3. 225oC

  4. 121oC

  5. 273oC

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

A steam temperature of 121oC is usually maintained for wet sterilisation in autoclave at 15 lbs/inch2. Culture media are wet sterilised because they are either liquid or semi-solid gels containing water.