Uranium-235 (92U235) is the primary isotope used for nuclear fission because it's fissile - it can sustain a chain reaction when struck by thermal neutrons. U-238 is the most abundant but not fissile, while U-237 and U-239 are not typically used for fission. Natural uranium contains only about 0.7% U-235, which is why enrichment is necessary for most reactor fuel and weapons.