C4 plants use phosphoenolpyruvate (PEP) as the primary CO2 acceptor in mesophyll cells, whereas C3 plants use ribulose-1,5-bisphosphate (RuBP) directly in the Calvin cycle. The end products of both pathways are ultimately carbohydrates, and both use similar photosynthetic pigments. The key difference is the initial CO2 fixation mechanism and the spatial separation of carbon fixation in C4 plants.