Multiple choice Bacteria conjugative pili are absent in Rickettsia felis Bacillus subtilis E. coli Brevibacterium mcbrellneri Reveal answer Fill a bubble to check yourself B Correct answer Explanation Bacillus subtilis is a Gram-positive bacillus and pili are absent in Gram-positive bacteria. They possess pili-like proteins or Type 4 pilins which assemble into pili (long polymeric fibers that function in bacterial adhesion and motility).