Multiple choice Preganglionic sympathetic fibres are adrenergic cholinergic Both (1) and (2) None of these Reveal answer Fill a bubble to check yourself B Correct answer Explanation Preganglionic sympathetic fibers release acetylcholine at their synapses with postganglionic neurons, making them cholinergic. This is true for all autonomic preganglionic fibers regardless of whether they are sympathetic or parasympathetic.