Which of the following sets represents selectable markers for Escherichia coli?
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Which of the following sets represents selectable markers for Escherichia coli?
Chloramphenicol, ampicillin, ranitidine
Tetracycline, ranitidine, cimetidine
Ampicillin, chloramphenicol, tetracycline
Chloramphenicol, streptomycin, kanamycin
Chloramphenicol, streptomycin, ranitidine
A gene whose expression allows one to identify cells that have been transformed or transfected with a vector containing the marker gene is known as a selectable marker which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc. are considered as useful selectable markers for Escherichia coli. Hence, the set ‘ampicillin, chloramphenicol, tetracycline’ represents selectable marker for Escherichia coli.