Multiple choice

How can an investigator scan a polypeptide sequence for potential membrane spanning segment?

  1. By calculating the hydropathy index for successive segment

  2. By determining the relative polarity of each amino acid

  3. By the presence of Tyr and Trp residues

  4. By the mild treatment that interfere with electrostatic interaction

  5. None of these
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A Correct answer
Explanation

The overall hydrophobicity of a sequence of amino acids is estimated by summing the free energies of transfer for the residues in the sequence, which yields a hydropathy index for that region.

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