Multiple choice

There are numerous techniques in cytogenetics to investigate the different features of chromosomes within a cell. Which of the following is a frequently used fluorescent pattern acquired employing quinacrine for staining the chromosomes?

  1. C-banding

  2. R-banding

  3. G-banding

  4. Q-banding

  5. T-banding

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Q-banding is a fluorescent pattern obtained using quinacrine for staining. The pattern of bands is very similar to that seen in G-banding. They can be recognised by a yellow fluorescence of differing intensity. Most part of the stained DNA is heterochromatin. Quinacrin (atebrin) binds both regions rich in AT and in GC, but only the AT-quinacrin-complex fluoresces.