There are numerous techniques in cytogenetics to investigate the different features of chromosomes within a cell. Which of the following is a frequently used fluorescent pattern acquired employing quinacrine for staining the chromosomes?
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C-banding
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R-banding
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G-banding
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Q-banding
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T-banding
D
Correct answer
Explanation
Q-banding is a fluorescent pattern obtained using quinacrine for staining. The pattern of bands is very similar to that seen in G-banding. They can be recognised by a yellow fluorescence of differing intensity. Most part of the stained DNA is heterochromatin. Quinacrin (atebrin) binds both regions rich in AT and in GC, but only the AT-quinacrin-complex fluoresces.