Multiple choice

Which of the following vectors has the highest DNA insert capacity?

  1. Plasmids

  2. Phages

  3. Cosmids

  4. Yeast artificial chromosomes

  5. Bacterial artificial chromosomes
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Yeast artificial chromosomes (YACs) can accommodate the largest DNA inserts, ranging from hundreds of kilobases to over 2 megabases. This far exceeds the capacity of BACs (100-200 kb), cosmids (35-45 kb), phages (5-25 kb), or plasmids (typically <15 kb). YACs are essential for cloning large genomic fragments.

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