Multiple choice

The step that initiates the process of 'Southern Blot Technique' is

  1. fragmentation of DNA by restriction of endonucleases

  2. electrophoresis of cut pieces on agarose gel

  3. isolation of DNA from suspected cell

  4. denaturation of DNA by treating it with NaOH

  5. transfer to a membrane

Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Southern blot begins with DNA isolation from cells - you cannot fragment, separate, or transfer DNA that hasn't been extracted first. After isolation (C), the DNA is cut with restriction enzymes (A), separated by electrophoresis (B), denatured (D), and transferred to a membrane (E). Option C is logically first - you need the DNA material before any processing.

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