Multiple choice The hardest substance of human body is enamel of incisors enamel of canines enamel of premolars all of the above Reveal answer Fill a bubble to check yourself D Correct answer Explanation Enamel is the hardest substance in the human body, rating 5 on the Mohs scale. It covers all teeth - incisors, canines, premolars, and molars. Therefore, saying 'all of the above' is correct because enamel on any tooth type is the hardest substance.