Multiple choice 2-phosphoglyceric acid is converted into phosphoenol pyruvic acid by _________. aldolase kinase enolase dehydrogenase Reveal answer Fill a bubble to check yourself C Correct answer Explanation Enolase converts 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP) by removing a water molecule (dehydration). This is the second-to-last step of glycolysis. PEP then donates its phosphate to ADP via pyruvate kinase.