Multiple choice How many NADH2 is/are formed in glycolysis? 2 3 1 4 Reveal answer Fill a bubble to check yourself A Correct answer Explanation Glycolysis produces a net gain of 2 NADH molecules per glucose. The NADH is formed during the oxidation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, with one NADH produced per each of the two 3-carbon molecules.