Multiple choice

Which of the following techniques is used to detect specific proteins in a sample of tissue homogenate or extract?

  1. Southern blot

  2. Western blot

  3. Northern blot

  4. Gel electrophoresis
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B Correct answer
Explanation

The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein.

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